SMAD2 Human
Description
Product Specs
Product Science Overview
SMAD2 belongs to the SMAD family of proteins, which are signal transducers and transcriptional modulators. These proteins mediate the signals from TGF-β and activin type 1 receptor kinases. SMAD2 contains two main domains:
- MH1 (MAD homology 1) domain: Involved in DNA binding.
- MH2 (MAD homology 2) domain: Responsible for protein-protein interactions and transcriptional activation.
Upon activation by TGF-β, SMAD2 undergoes phosphorylation, which leads to its dissociation from the SMAD anchor for receptor activation (SARA) protein. The phosphorylated SMAD2 then forms a complex with SMAD4, which translocates into the nucleus to regulate the transcription of target genes .
Mutations or dysregulation of SMAD2 have been associated with several diseases, including:
- Loeys-Dietz Syndrome 6: A connective tissue disorder characterized by aortic aneurysms and other cardiovascular abnormalities.
- Congenital Heart Defects, Multiple Types, 8, With Or Without Heterotaxy: A group of heart defects present at birth .
Additionally, SMAD2 may act as a tumor suppressor in colorectal carcinoma by regulating the transcription of genes involved in cell cycle control and apoptosis .
Recombinant SMAD2 is produced using E. coli expression systems and is often tagged with His and Flag tags for purification and detection purposes. The recombinant protein is typically lyophilized and can be reconstituted for use in various research applications, including:
- Signal transduction studies
- Protein-protein interaction assays
- Transcriptional regulation experiments
The recombinant SMAD2 protein is stable for up to 12 months when stored at -20 to -80°C and can be used to study the molecular mechanisms of TGF-β signaling and its role in disease .
