SLAMF1 Human, Sf9
Sf9, Baculovirus cells.
Greater than 95.0% as determined by SDS-PAGE.
Description
SLAMF1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 226 amino acids (21-237a.a.) and having a molecular mass of 25.3kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
SLAMF1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Sf9, Baculovirus cells.
ADPASYGTGG RMMNCPKILR QLGSKVLLPL TYERINKSMN KSIHIVVTMA KSLENSVENK IVSLDPSEAG PPRYLGDRYK FYLENLTLGI RESRKEDEGW YLMTLEKNVS VQRFCLQLRL YEQVSTPEIK VLNKTQENGT CTLILGCTVE KGDHVAYSWS EKAGTHPLNP ANSSHLLSLT
LGPQHADNIY ICTVSNPISN NSQTFSPWPG CRTDPSETKP HHHHHH.
Product Science Overview
The SLAMF1 gene is located on human chromosome 1 and consists of eight exons and seven introns . The gene undergoes alternative splicing, resulting in several isoforms of the protein, including the conventional transmembrane isoform (mCD150), secreted isoform (sCD150), cytoplasmic isoform (cCD150), and the novel transmembrane isoform (nCD150) . SLAMF1 is expressed in various hematopoietic cells, including thymocytes, natural killer T (NKT) cells, T cells, B cells, monocytes, macrophages, and dendritic cells . Its expression is also detected in platelets .
SLAMF1 is a type I transmembrane protein with a molecular weight between 70 kDa and 95 kDa . The extracellular region of the receptor comprises one immunoglobulin variable-like domain and one immunoglobulin constant 2-like domain . The intracellular region contains two intracellular tyrosine-based switch motifs (ITSMs) that interact with SH2 domain-containing proteins . However, the intracellular region of the novel transmembrane isoform (nCD150) lacks ITSMs .
SLAMF1 is involved in various immune functions, including the regulation of thymocyte development, T cell proliferation, differentiation, and T cell function . It plays a role in the cytotoxic activity of CD8+ T cells and the production of cytokines such as interleukin-4 (IL-4), interleukin-13 (IL-13), and interferon-gamma (IFNγ) . SLAMF1 also promotes Th1-protective responses during infections, such as tuberculosis .
SLAMF1 has been shown to enhance the internalization of Mycobacterium tuberculosis by macrophages . It interacts with the bacteria and colocalizes with early and late endosomes/lysosomes markers, suggesting its involvement in the endolysosomal maturation process . Increased levels of SLAMF1 have been detected in CD14 cells from pleural effusions of tuberculosis patients, indicating its active function at the site of infection .
Recombinant SLAMF1 (Human, Sf9) refers to the human SLAMF1 protein produced using the Sf9 insect cell expression system. This system is commonly used for the production of recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells. The recombinant SLAMF1 protein can be used in various research applications, including studies on immune regulation, receptor-ligand interactions, and the development of therapeutic agents targeting SLAMF1.
