SIAH1 Human

Siah E3 Ubiquitin Protein Ligase 1 Human Recombinant
Cat. No.
BT5011
Source
E.coli.
Synonyms
Siah E3 ubiquitin protein ligase 1, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), E3 ubiquitin-protein ligase SIAH1, HUMSIAH, hSIAH1, Siah-1a, SIAH1A, EC 6.3.2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SIAH1 Human Recombinant produced in E. coli is a single polypeptide chain containing 216 amino acids (90-282) and having a molecular mass of 24.1 kDa.
SIAH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The E3 ubiquitin-protein ligase SIAH1, also known as SIAH1, is a member of the SIAH family, which stands for seven in absentia homolog. It acts as a tumor suppressor protein and is found in the intestinal epithelium, becoming active during apoptosis. SIAH1 participates in the ubiquitination and subsequent proteasome-mediated degradation of specific proteins. This protein consists of two main domains: an N-terminal RING-finger domain essential for proteolysis and a cysteine-rich C-terminal domain that regulates both oligomerization and the binding of SIAH1 to its target proteins. Notably, SIAH1 indirectly degrades beta-catenin by forming a complex with Siah-interacting protein (SIP), Skp1, and Ebi.
Description
Recombinant SIAH1 protein for human use is produced in E. coli. It is a single polypeptide chain with a molecular weight of 24.1 kDa, comprised of 216 amino acids (residues 90-282). This SIAH1 protein is fused to a 23 amino acid His-tag located at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The SIAH1 solution is provided at a concentration of 0.25mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 100mM NaCl, 1mM DTT, and 40% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the solution at -20°C. To maximize long-term stability, we recommend adding a carrier protein such as HSA or BSA (0.1%). Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of this product is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Siah E3 ubiquitin protein ligase 1, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), E3 ubiquitin-protein ligase SIAH1, HUMSIAH, hSIAH1, Siah-1a, SIAH1A, EC 6.3.2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSVANSVLF PCKYASSGCE ITLPHTEKAD HEELCEFRPY SCPCPGASCK WQGSLDAVMP HLMHQHKSIT TLQGEDIVFL ATDINLPGAV DWVMMQSCFG FHFMLVLEKQ EKYDGHQQFF AIVQLIGTRK QAENFAYRLE LNGHRRRLTW EATPRSIHEG IATAIMNSDC LVFDTSIAQL FAENGNLGIN VTISMC.

Product Science Overview

Introduction

Siah E3 Ubiquitin Protein Ligase 1, commonly referred to as Siah1, is a human homolog of the Drosophila seven in absentia (sina) gene. It is a member of the E3 ubiquitin ligase family, which plays a crucial role in the ubiquitination process, a post-translational modification that tags proteins for degradation by the proteasome .

Structure and Function

Siah1 is encoded by the SIAH1 gene located on chromosome 16 in humans . The protein is involved in the ubiquitination and proteasome-mediated degradation of specific proteins. This activity is essential for various cellular processes, including cell cycle regulation, apoptosis, and response to hypoxia .

Biological Significance

Siah1 has been implicated in several critical biological pathways and diseases. It acts as a tumor suppressor gene and plays a significant role in the development of malignant tumors, such as colorectal cancer (CRC). Studies have shown that downregulation of Siah1 promotes CRC cell proliferation and migration by regulating the ubiquitination and degradation of key signaling proteins like AKT and YAP .

Additionally, Siah1 is involved in the regulation of the cellular response to hypoxia and the induction of apoptosis. Its activity has also been linked to the development of certain forms of Parkinson’s disease .

Research and Clinical Implications

The potential role of Siah1 in cancer progression and its involvement in critical signaling pathways make it a promising target for therapeutic interventions. Overexpression of Siah1 has been shown to inhibit CRC cell proliferation and invasion, suggesting its potential as a prognostic biomarker and therapeutic target in cancer treatment .

Moreover, understanding the molecular mechanisms of Siah1’s activity can provide insights into the development of novel therapeutic strategies for diseases associated with its dysregulation, such as Parkinson’s disease and other neurodegenerative disorders .

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