SHMT1 Antibody

Serine Hydroxymethyltransferase 1 (SHMT1) is a crucial enzyme involved in the interconversion of serine and glycine. This enzyme is encoded by the SHMT1 gene and is primarily located in the cytoplasm. SHMT1 plays a significant role in the folate cycle, which is essential for the synthesis of nucleotides and amino acids .

SHMT1 catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction is vital for providing one-carbon units necessary for the synthesis of methionine, thymidylate, and purines in the cytoplasm . The enzyme’s activity is crucial for cellular proliferation and DNA synthesis, making it an important target for cancer research and treatment.

The SHMT1 gene is located on chromosome 17 within the Smith-Magenis syndrome region. It has multiple transcript variants due to alternative splicing. A pseudogene of SHMT1 is also present on the short arm of chromosome 1 .

Mouse anti-human SHMT1 antibodies are monoclonal antibodies derived from hybridization of mouse myeloma cells with spleen cells from immunized mice. These antibodies are used in various applications, including Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry (FC) .

• Western Blotting (WB): Used to detect SHMT1 in various lysates, including human, mouse, and rat samples .
• Immunohistochemistry (IHC): Applied to paraffin-embedded tissue sections to visualize SHMT1 expression .
• Immunofluorescence (IF): Utilized for staining fixed cells to observe SHMT1 localization .
• Flow Cytometry (FC): Employed to analyze intracellular SHMT1 levels in cell suspensions .

SHMT1 is a key enzyme in the folate cycle, and its dysregulation is associated with various diseases, including cancer and metabolic disorders. The mouse anti-human SHMT1 antibody is a valuable tool for researchers studying the enzyme’s function, expression, and role in disease mechanisms .