SGK1 Human
Serum/Glucocorticoid Regulated Kinase 1 Human Recombinant
Cat. No.
BT14625
Source
Escherichia Coli.
Synonyms
Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1, SGK1, SGK.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SGK1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 393 amino acids (60-431 a.a.) and having a molecular mass of 44.5kDa.
SGK1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SGK1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Serum-and glucocorticoid-regulated kinase 1 (SGK1), a member of the "AGC" subfamily of serine/threonine protein kinases (which includes protein kinases A, G, and C), plays a crucial role in activating potassium, sodium, and chloride channels. This suggests its involvement in regulating cell survival, neuronal excitability, and renal sodium excretion. SGK1 is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK-1) and in vivo by signals that activate phosphatidylinositol (PI) 3-kinase.
Description
Recombinant human SGK1, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 393 amino acids (residues 60-431). With a molecular mass of 44.5 kDa, this protein is fused to a 21 amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The SGK1 protein solution (0.25 mg/mL) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 30% glycerol, 0.2 M NaCl, 2 mM DTT, and 0.1 mM PMSF.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the SGK1 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1, SGK1, SGK.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPF LVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTV DWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL.
Product Science Overview
Discovery and Structure
Regulation and Activation
Functions and Roles
SGK1 is involved in a wide range of cellular functions, including:
- Ion Channel and Transporter Regulation: SGK1 regulates various ion channels and transporters, which are essential for maintaining cellular homeostasis .
- Cell Proliferation and Apoptosis: It plays a role in cell growth and programmed cell death, making it significant in cancer research .
- Immune and Inflammatory Responses: SGK1 modulates immune cells such as T cells, macrophages, dendritic cells, and neutrophils, and is involved in the pathogenesis of immune and inflammatory diseases .
- Memory Formation and Long-term Potentiation: SGK1 is also implicated in cognitive functions, including memory formation .
Clinical Significance
SGK1 has been associated with several human diseases, including:
- Cancer: It is involved in the development of various cancers, such as cervical cancer and prostate cancer .
- Fibrotic Diseases: SGK1 plays a role in fibrotic conditions like pulmonary fibrosis and liver cirrhosis .
- Neurodegenerative Diseases: It has been linked to Alzheimer’s disease and other neurodegenerative disorders .
- Metabolic Disorders: SGK1 is also associated with type 2 diabetes mellitus and hypertension .
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