SGCB Human

Sarcoglycan Beta Human Recombinant
Cat. No.
BT15765
Source
Escherichia Coli.
Synonyms
Beta-sarcoglycan, Beta-SG, 43 kDa dystrophin-associated glycoprotein, 43DAG, A3b, LGMD2E, Sarcoglycan, Beta (43kDa Dystrophin-Associated Glycoprotein), Limb Girdle Muscular Dystrophy 2E (Non-Linked Families), SGC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SGCB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (87-318 a.a) and having a molecular mass of 27.8kDa.
SGCB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Sarcoglycan Beta, also called SGCB, is a member of the sarcoglycan protein family. It is a component of the sarcoglycan complex, which itself is a subcomplex of the larger dystrophin-glycoprotein complex. This complex plays a crucial role in linking the intracellular actin cytoskeleton to the extracellular matrix.
Description
Recombinant human SGCB, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 255 amino acids (spanning from position 87 to 318), resulting in a molecular weight of 27.8 kDa. For purification and detection purposes, a 23 amino acid His-tag is fused to the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The SGCB protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is buffered with 20mM Tris-HCl at pH 8.0 and contains 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, adding a carrier protein (either 0.1% HSA or BSA) is advisable. Importantly, repeated freezing and thawing of the protein should be avoided.
Purity
The purity of SGCB is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Beta-sarcoglycan, Beta-SG, 43 kDa dystrophin-associated glycoprotein, 43DAG, A3b, LGMD2E, Sarcoglycan, Beta (43kDa Dystrophin-Associated Glycoprotein), Limb Girdle Muscular Dystrophy 2E (Non-Linked Families), SGC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSWAVIRIG PNGCDSMEFH ESGLLRFKQV SDMGVIHPLY KSTVGGRRNE NLVITGNNQP IVFQQGTTKL SVENNKTSIT SDIGMQFFDP RTQNILFSTD YETHEFHLPS GVKSLNVQKA STERITSNAT SDLNIKVDGR AIVRGNEGVF IMGKTIEFHM GGNMELKAEN SIILNGSVMV STTRLPSSSS GDQLGSGDWV RYKLCMCADG TLFKVQVTSQ NMGCQISDNP CGNTH.

Product Science Overview

Introduction

Sarcoglycan Beta (SGCB) is a crucial component of the dystrophin-glycoprotein complex (DGC), which plays a significant role in maintaining the structural integrity of muscle cells. The recombinant form of this protein is often used in research to study its function and role in various muscular dystrophies.

Gene and Protein Structure

The SGCB gene is located on chromosome 4q12 and consists of 6 exons spanning approximately 13.5 kb of genomic DNA . The gene encodes a protein of 318 amino acids with a calculated molecular mass of 34.8 kDa . The protein structure includes:

  • A short N-terminal intracellular domain
  • A transmembrane domain
  • A large C-terminal extracellular domain

The intracellular domain contains a putative serine phosphorylation site, while the extracellular domain has three putative N-glycosylation sites and five cysteines that may participate in disulfide bond formation .

Expression and Function

Beta-sarcoglycan is ubiquitously expressed, with predominant expression in muscle tissues, including the heart and skeletal muscles . It colocalizes with the DGC at the sarcolemma, providing a structural linkage between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells .

Role in Muscular Dystrophy

Mutations in the SGCB gene are associated with autosomal recessive limb-girdle muscular dystrophy type 4 (LGMDR4) . These mutations can lead to the disruption of the DGC, resulting in muscle weakness and degeneration. Studies have shown that recombinant beta-sarcoglycan can be used to investigate the molecular mechanisms underlying these muscular dystrophies .

Recombinant Protein

Recombinant human beta-sarcoglycan is produced using various expression systems, such as wheat germ . The recombinant protein is often tagged (e.g., GST tag) to facilitate purification and detection in experimental assays. It is validated for use in techniques such as SDS-PAGE, Western blotting (WB), and ELISA .

Applications in Research

The recombinant form of beta-sarcoglycan is valuable in research for:

  • Studying the protein’s role in the DGC and muscle cell integrity
  • Investigating the effects of mutations on protein function and stability
  • Developing potential therapeutic approaches for muscular dystrophies

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