SF3B14 Human

Splicing Factor 3B, 14 kDa Subunit Human Recombinant
Cat. No.
BT15468
Source
Escherichia Coli.
Synonyms
Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, SF3B14, CGI-110, HSPC175, HT006, P14, SAP14, SF3B14a.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SF3B14 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids (1-125a.a.) and having a molecular mass of 16.7kDa. The SF3B14 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
SF3B14, a nuclear protein comprising 125 amino acids, is an integral part of the splicing factor 3b complex. This complex plays a crucial role in the splicing of pre-mRNA by interacting with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (specifically, U2 snRNP) within spliceosomes. SF3B14 is essential for pre-mRNA splicing by entering the spliceosome and binding to the pre-mRNA branch site. This binding facilitates the interaction of snRNP with the branch sites of U2 and U12 present in the 17S U2 and 18S U11/U12 snRNP complexes.
Description
Human Recombinant SF3B14, expressed in E. coli, is fused with a 20 amino acid His tag at its N-terminus. This results in a single, non-glycosylated polypeptide chain consisting of 145 amino acids (1-125a.a.) with a molecular weight of 16.7kDa. The purification of SF3B14 is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The SF3B14 solution is provided at a concentration of 0.5 mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA, and 30% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the SF3B14 solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To further enhance long-term stability, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of SF3B14 is determined to be greater than 95.0% based on SDS-PAGE analysis.
Synonyms
Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, SF3B14, CGI-110, HSPC175, HT006, P14, SAP14, SF3B14a.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK.

Product Science Overview

Introduction

Splicing Factor 3B, 14 kDa Subunit (SF3B14), also known as SF3B6, is a crucial component of the splicing factor 3b complex. This complex plays a significant role in the pre-mRNA splicing process, which is essential for the maturation of messenger RNA (mRNA) in eukaryotic cells. The SF3B14 subunit is a 14 kDa protein that interacts with other components of the splicing machinery to facilitate the accurate removal of introns from pre-mRNA transcripts.

Structure and Composition

The SF3B14 protein is a part of the larger SF3b complex, which consists of seven protein subunits with molecular sizes ranging from 10 to 155 kDa . The SF3b complex, along with the SF3a complex and a 12S RNA unit, forms the U2 small nuclear ribonucleoprotein (snRNP) complex. This complex is integral to the spliceosome, the molecular machine responsible for splicing pre-mRNA .

Function

SF3B14 is involved in the recognition of the branch point sequence (BPS) within the pre-mRNA. This recognition is crucial for the assembly and activation of the spliceosome. The SF3b complex, including SF3B14, binds to the pre-mRNA upstream of the intron’s branch site in a sequence-independent manner, anchoring the U2 snRNP to the pre-mRNA . This interaction ensures the precise removal of introns and the ligation of exons, resulting in the formation of mature mRNA.

Recombinant SF3B14

Recombinant SF3B14 is produced using various expression systems, such as Escherichia coli (E. coli). The recombinant protein is often tagged with a His-ABP tag for purification purposes and is supplied in a liquid form with a concentration of at least 5.0 mg/mL . This recombinant protein is used in various research applications, including blocking assays and control experiments .

Applications in Research

The recombinant SF3B14 protein is widely used in molecular biology research to study the mechanisms of pre-mRNA splicing. It serves as a valuable tool for investigating the interactions between different components of the splicing machinery and for elucidating the molecular details of the splicing process. Additionally, recombinant SF3B14 can be used in blocking experiments to validate the specificity of antibodies targeting the SF3B14 protein .

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