SETD7 Antibody

Set7/9, also known as SETD7, is a lysine-specific histone methyltransferase that plays a crucial role in the regulation of gene expression through post-translational modifications (PTMs). This enzyme is responsible for the methylation of histone H3 at lysine 4 (H3K4), a modification associated with transcriptional activation .

Set7/9 was first identified and characterized in 2001 by Zhang and Reinberg’s labs . The enzyme contains a SET domain, which is a conserved sequence found in all organisms, responsible for the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to the lysine residues of protein substrates .

Set7/9 is involved in the methylation of both histone and non-histone proteins. The methylation of histone H3 at lysine 4 (H3K4me) by Set7/9 is associated with transcriptional activation, as it creates a chromatin environment conducive to gene expression . Additionally, Set7/9 methylates several non-histone proteins, including p53, pRb, YAP, DNMT1, SOX2, and FOXO3, which are crucial regulators of cellular processes such as stemness, differentiation, and development .

Set7/9 plays a significant role in various cellular processes, including:

• Gene Expression Regulation: By methylating histone H3 at lysine 4, Set7/9 facilitates the transcriptional activation of genes .
• Cell Cycle Regulation: Set7/9 methylates non-histone proteins involved in cell cycle regulation, influencing cell proliferation and differentiation .
• Stemness and Differentiation: Set7/9 is crucial for maintaining the balance between cellular stemness and differentiation, which is essential for the development of multicellular organisms .
• Pathological Processes: Aberrant activity of Set7/9 has been linked to pathological conditions, including the formation of cancer stem cells .

The mouse anti-human Set7/9 antibody is raised in mice using recombinant human Set7/9 as the immunogen . This antibody is used in various research applications to study the expression and function of Set7/9 in human cells.