SET Human
SET Human Recombinant
Cat. No.
BT15382
Source
Escherichia Coli.
Synonyms
2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA, Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, Template-activating factor I, SET.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
SET Human Recombinant produced in E. coli is a single polypeptide chain containing 313 amino acids (1-290) and having a molecular mass of 35.9kDa. SET is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
The protein SET, primarily found in the endoplasmic reticulum, also exists in the nucleus. It plays a role in inhibiting apoptosis induced by cytotoxic T lymphocytes and can enhance DNA replication of the adenovirus genome. SET functions by inhibiting histone acetylation, particularly of histone H4, by masking histone lysines from histone acetylases (HAT).
Description
Recombinant SET protein, of human origin, is produced in E. coli. It consists of a single polypeptide chain of 290 amino acids (residues 1-290) with a molecular weight of 35.9kDa. A 23 amino acid His-tag is fused to the N-terminus to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The SET protein solution is provided at a concentration of 0.5mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the solution should be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
Purity is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA, Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, Template-activating factor I, SET.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD.
Product Science Overview
Structure and Characteristics
SET Human Recombinant is a single polypeptide chain containing 313 amino acids, with a molecular mass of approximately 35.9 kDa. It is fused to a 23 amino acid His-tag at the N-terminus, which facilitates its purification using chromatographic techniques . The protein is typically provided as a sterile, filtered clear solution, formulated in a buffer containing Tris-HCl, NaCl, glycerol, and DTT to maintain its stability .
Biological Functions
SET is a multifunctional protein involved in several critical cellular processes:
- Inhibition of Apoptosis: SET inhibits apoptosis, the programmed cell death, following an attack by cytotoxic T lymphocytes. This function is crucial for cell survival under immune stress .
- Enhancement of DNA Replication: SET enhances the DNA replication of the adenovirus genome, indicating its role in viral replication and possibly in cellular DNA replication .
- Inhibition of Histone Acetylation: SET inhibits the acetylation of nucleosomes, particularly histone H4, by histone acetylases (HAT). This inhibition is achieved by masking histone lysines, preventing their acetylation and thereby influencing gene expression .
Industrial Production
The production of SET Human Recombinant involves several steps:
- Gene Cloning: The human SET gene is cloned into an expression vector, which is then introduced into E. coli cells.
- Protein Expression: The E. coli cells are cultured under conditions that induce the expression of the SET protein.
- Purification: The protein is purified using chromatographic techniques, leveraging the His-tag for affinity purification .
Applications
SET Human Recombinant has various applications in research and biotechnology:
- Study of Apoptosis: Researchers use SET to study the mechanisms of apoptosis and its inhibition.
- Gene Expression Regulation: SET is used to investigate the regulation of gene expression through histone modification.
- Viral Replication: The role of SET in enhancing viral DNA replication makes it a valuable tool in virology research.
Storage and Stability
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