SERPINB3 Human
Serpin Peptidase Inhibitor, Clade B Member 3 Human Recombinant
Cat. No.
BT24153
Source
Escherichia Coli.
Synonyms
Serpin B3, Squamous cell carcinoma antigen 1, Protein
T4-A, SCCA-1, Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3,
SCCA1, Serine (Or Cysteine) Proteinase Inhibitor, Clade B
(Ovalbumin), Member 3, Squamous Cell Carcinoma Antigen 1, Protein T4-A, SCCA-1,
Serpin B3, HsT1196, SCCA-PD, T4-A , SCCA, SCC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
SERPINB3 Human Recombinant produced in E.Coli is a
single, non-glycosylated polypeptide chain containing 413 amino acids (1-390 a.a)
and having a molecular mass of 47kDa.
SERPINB3 is fused to a 23 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Serpin Peptidase Inhibitor, Clade B Member 3, also known as SERPINB3, is a papain-like cysteine protease inhibitor that modulates the host immune response against tumor cells. The SERPINB3 gene encodes a protein that inhibits UV-induced apoptosis by suppressing the activity of c-Jun NH(2)-terminal kinase (JNK1).
Description
SERPINB3 Human Recombinant protein, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 413 amino acids (1-390 a.a.) with a molecular mass of 47 kDa. It is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
SERPINB3 protein solution (0.5 mg/ml) in Phosphate buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 90.0% purity as determined by SDS-PAGE.
Synonyms
Serpin B3, Squamous cell carcinoma antigen 1, Protein
T4-A, SCCA-1, Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3,
SCCA1, Serine (Or Cysteine) Proteinase Inhibitor, Clade B
(Ovalbumin), Member 3, Squamous Cell Carcinoma Antigen 1, Protein T4-A, SCCA-1,
Serpin B3, HsT1196, SCCA-PD, T4-A , SCCA, SCC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNSLSEA NTKFMFDLFQ QFRKSKENNI FYSPISITSA LGMVLLGAKD
NTAQQIKKVL HFDQVTENTT GKAATYHVDR SGNVHHQFQK LLTEFNKSTD AYELKIANKL FGEKTYLFLQ
EYLDAIKKFY QTSVESVDFA NAPEESRKKI NSWVESQTNE KIKNLIPEGN IGSNTTLVLV NAIYFKGQWE
KKFNKEDTKE EKFWPNKNTY KSIQMMRQYT SFHFASLEDV QAKVLEIPYK GKDLSMIVLL PNEIDGLQKL
EEKLTAEKLM EWTSLQNMRE TRVDLHLPRF KVEESYDLKD TLRTMGMVDI FNGDADLSGM TGSRGLVLSG
VLHKAFVEVT EEGAEAAAAT AVVGFGSSPT STNEEFHCNH PFLFFIRQNK TNSILFYGRF SSP.
Product Science Overview
Gene and Protein Structure
The SERPINB3 gene encodes a protein that consists of 390 amino acids . It belongs to the serine protease inhibitor family, which functions by inhibiting proteases through a suicide substrate mechanism. Upon proteolytic cleavage of their reactive site loop, serpins undergo a conformational rearrangement that traps the covalently attached protease in an inactive form .
Biological Functions
SERPINB3 plays a crucial role in various biological processes, including:
- Inhibition of Proteases: It acts as a cysteine protease inhibitor, modulating the host immune response against tumor cells .
- Regulation of Apoptosis: It functions as an inhibitor of UV-induced apoptosis by suppressing the activity of c-Jun NH2-terminal kinase (JNK1) .
- Disease Progression and Cancer: SERPINB3 is involved in increasing fibrosis, cell proliferation, and invasion, besides conferring resistance to apoptosis .
Clinical Significance
SERPINB3 is clinically significant as a tumor marker for squamous cell carcinoma (SCC). It is detected in the cytoplasm of normal and some malignant squamous cells. Elevated levels of SERPINB3 are associated with various types of squamous cell carcinoma, including those of the cervix, head and neck, lung, and esophagus . The protein’s levels fall after tumor resection and rise in patients with recurrent disease .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
