SEPW1 Human

Selenoprotein W 1 Human Recombinant

Cat. No.

BT21743

Source

Escherichia Coli.

Synonyms

Selenoprotein W, 1, SelW, Selenoprotein W.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

SEPW1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids (1-87 a.a) and having a molecular mass of 11.8kDa.

SEPW1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Selenoprotein W 1, also called SEPW1, is a selenoprotein containing a selenocysteine (Sec) residue at its active site. This selenocysteine is encoded by the UGA codon, typically a stop codon for translation. However, the 3' UTR of selenoprotein genes possesses a common stem-loop structure known as the sec insertion sequence (SECIS). SECIS enables the recognition of UGA as a Sec codon rather than a stop signal. SEPW1 is most abundantly expressed in skeletal muscle and heart tissues. It plays a role in oxidation-reduction reactions.

Description

Recombinant human SEPW1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 110 amino acids (amino acids 1-87). It has a molecular weight of 11.8 kDa. SEPW1 is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.

Physical Appearance

A clear, sterile-filtered solution.

Formulation

The SEPW1 protein solution has a concentration of 0.25 mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.

Stability

For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity exceeds 95.0%, as determined by SDS-PAGE analysis.

Synonyms

Selenoprotein W, 1, SelW, Selenoprotein W.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMALAVRV VYCGACGYKS KYLQLKKKLE DEFPGRLDIC GEGTPQATGF FEVMVAGKLI HSKKKGDGYV DTESKFLKLV AAIKAALAQG.

Product Science Overview

Introduction

Selenoprotein W 1 (SEPW1) is a small selenoprotein that plays a crucial role in various biological processes. It is a member of the selenoprotein family, which contains the amino acid selenocysteine. Selenocysteine is known as the 21st amino acid and is unique due to its incorporation of selenium, an essential trace element. SEPW1 is widely distributed in various tissues and has been the subject of extensive research due to its potential implications in health and disease.

Structure and Function

SEPW1 is characterized by its small size, approximately 9.5 kDa, and contains a Cys-X-X-Sec motif, where “Sec” stands for selenocysteine. This motif is critical for the protein’s function. The presence of selenocysteine allows SEPW1 to participate in redox reactions, which are essential for maintaining cellular homeostasis and protecting cells from oxidative damage.

Biological Role

The primary function of SEPW1 is related to its antioxidant properties. It helps in the detoxification of reactive oxygen species (ROS) and maintains redox balance within cells. This function is vital for preventing cellular damage and ensuring proper cellular function. SEPW1 is also involved in various other biological processes, including signal transduction, cell proliferation, and immune response.

Expression and Regulation

SEPW1 expression is regulated at both the transcriptional and post-transcriptional levels. The expression of SEPW1 mRNA and protein is correlated, and it is widely distributed across different tissues. The regulation of SEPW1 is influenced by selenium availability, as selenium is a critical component of selenocysteine. Selenium deficiency can lead to reduced expression of SEPW1 and other selenoproteins, which can have significant health implications.

Health Implications

Research has shown that SEPW1 and other selenoproteins play a crucial role in human health. Selenium deficiency has been linked to various health issues, including immune dysfunction, cardiovascular diseases, and certain types of cancer. SEPW1, due to its antioxidant properties, is believed to protect against oxidative stress-related diseases. Additionally, SEPW1 has been implicated in muscle function and development, making it an essential protein for overall health.

Recombinant SEPW1

Recombinant SEPW1 is produced using biotechnological methods to study its structure, function, and potential therapeutic applications. By expressing SEPW1 in a controlled environment, researchers can investigate its properties in detail and explore its potential use in treating diseases related to oxidative stress and selenium deficiency.

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SEPW1 Human

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