SEPSECS Human

Selenocysteinyl-tRNA(Sec) synthase Human Recombinant
Cat. No.
BT24978
Source
Escherichia Coli.
Synonyms
SLA, SLA/LP autoantigen, SLA-p35, Selenocysteinyl-tRNA(Sec) synthase, Selenocysteine synthase, Liver-pancreas antigen, Soluble liver antigen, Sec synthase, UGA suppressor tRNA-associated protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SEPSECS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 521 amino acids (1-501a.a.) and having a molecular mass of 57.9kDa.
SEPSECS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
SEPSECS is an enzyme that plays a crucial role in the synthesis of selenocysteine, an amino acid. It facilitates the conversion of O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) by utilizing selenophosphate as the selenium donor. This enzyme is particularly important in the context of autoimmune hepatitis, where it serves as a specific marker.
Description
Produced in E. coli, our SEPSECS is a single, non-glycosylated polypeptide chain consisting of 521 amino acids (specifically, amino acids 1 through 501). It has a molecular weight of 57.9 kDa. For purification purposes, a 20 amino acid His-tag is attached to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized through filtration.
Formulation
The SEPSECS protein is provided in a solution at a concentration of 0.5 mg/ml. The formulation buffer consists of 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 100 mM NaCl, and 10% glycerol.
Purity
The purity of SEPSECS is greater than 90%, as determined by SDS-PAGE analysis.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For longer storage, freezing at -20°C is recommended. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It's important to avoid repeated freeze-thaw cycles.
Synonyms
SLA, SLA/LP autoantigen, SLA-p35, Selenocysteinyl-tRNA(Sec) synthase, Selenocysteine synthase, Liver-pancreas antigen, Soluble liver antigen, Sec synthase, UGA suppressor tRNA-associated protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S.

Product Science Overview

Selenocysteine Biosynthesis

The biosynthesis of selenocysteine is a multi-step process that involves several key enzymes and cofactors. The process begins with the aminoacylation of tRNA(Sec) with serine by seryl-tRNA synthetase. This serine is then phosphorylated by O-phosphoseryl-tRNA kinase (PSTK) to form O-phosphoseryl-tRNA(Sec). The final step in the biosynthesis is catalyzed by SepSecS, which converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) through a tRNA-dependent mechanism .

Mechanism of Action

SepSecS operates through a pyridoxal phosphate-dependent mechanism. The enzyme forms a complex with tRNA(Sec), phosphoserine, and thiophosphate. The binding of tRNA(Sec) to SepSecS induces a conformational change in the enzyme’s active site, allowing the phosphoserine attached to tRNA(Sec) to be properly oriented for the reaction to occur. This reaction results in the formation of selenocysteinyl-tRNA(Sec), which is then used in the synthesis of selenoproteins .

Structural Insights

The crystal structure of human SepSecS in complex with tRNA(Sec) has provided significant insights into the enzyme’s function. The structure reveals that two tRNA(Sec) molecules bind to each SepSecS tetramer through their 13-base pair acceptor-TPsiC arm. This binding is essential for the proper orientation and catalysis of the reaction .

Biological Significance

Selenoproteins, which contain selenocysteine at their active sites, play critical roles in various biological processes, including antioxidant defense, thyroid hormone metabolism, and redox regulation. The proper functioning of SepSecS is therefore essential for the synthesis of these vital proteins and for maintaining cellular homeostasis .

Human Recombinant SepSecS

Recombinant human SepSecS is produced using advanced biotechnological methods, allowing for the study of its structure and function in detail. This recombinant enzyme is used in various research applications to understand the mechanisms of selenocysteine biosynthesis and the role of selenoproteins in human health and disease .

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