SENP8 Human

Sentrin Specific Peptidase Family Member 8 Human Recombinant
Cat. No.
BT24350
Source
Escherichia Coli.
Synonyms
SUMO/sentrin specific peptidase family member 8, DEN1, NEDP1, Protease cysteine 2
(NEDD8 specific), PRSC2, NEDD8-specific protease 1, HsT17512, Deneddylase-1, NEDD8 specific-protease cysteine 2, Sentrin/SUMO-specific protease SENP8.
Appearance
SENP8 is supplied as a sterile filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SENP8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-212) and having a molecular mass of 26.2 kDa.
The SENP8 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
SENP8, a member of the sentrin-specific protease family, is a cysteine protease that plays a crucial role in the processing and deconjugation of NEDD8, a ubiquitin-like protein also known as neural precursor cell expressed developmentally downregulated 8.
Description
Recombinant human SENP8, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 232 amino acids (residues 1-212). With a molecular weight of 26.2 kDa, this protein is engineered with a 20 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
SENP8 is provided as a clear, sterile solution that has been filtered for purity.
Formulation
SENP8 protein is supplied at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCL (pH 8), 0.1M NaCl, 1mM DTT, and 10% Glycerol.
Stability
For short-term storage (2-4 weeks), SENP8 should be stored at 4°C. For extended storage, it is recommended to store the protein frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of SENP8 is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
SUMO/sentrin specific peptidase family member 8, DEN1, NEDP1, Protease cysteine 2
(NEDD8 specific), PRSC2, NEDD8-specific protease 1, HsT17512, Deneddylase-1, NEDD8 specific-protease cysteine 2, Sentrin/SUMO-specific protease SENP8.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLITTLA KK

Product Science Overview

Gene and Protein Structure

The SENP8 gene is located on chromosome 15 in humans . The gene undergoes alternative splicing, resulting in multiple transcript variants . The encoded protein, SENP8, is involved in two essential functions within the NEDD8 pathway:

  1. Processing of full-length NEDD8 to its mature form.
  2. Deconjugation of NEDD8 from targeted proteins, such as cullins or p53 .
Biological Functions

SENP8’s primary function is to regulate the neddylation process, which involves the conjugation of NEDD8 to target proteins. This conjugation is essential for the optimal activity of ubiquitin ligases . SENP8 catalyzes the removal of NEDD8 from these proteins, a process known as deneddylation . This activity is critical for maintaining cellular homeostasis and regulating various cellular processes, including protein degradation, cell cycle progression, and signal transduction .

Clinical Significance

Mutations or dysregulation of the SENP8 gene have been associated with several diseases, including Kunjin Encephalitis and Prostate Malignant Phyllodes Tumor . The enzyme’s role in deubiquitination and protein metabolism pathways highlights its importance in cellular function and disease pathology .

Research and Applications

Recombinant human SENP8 is widely used in research to study the mechanisms of neddylation and deneddylation. Understanding SENP8’s function and regulation can provide insights into the development of therapeutic strategies for diseases associated with ubiquitin-like protein modifications .

For more detailed information, you can refer to the GeneCards entry for SENP8 or the Wikipedia page on SENP8.

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