SEMA7A Human
Sf9, Insect cells.
Semaphorin-7A, CDw108, JMH blood group antigen, John-Milton-Hargen human blood group Ag, Semaphorin-K1, Sema K1, Semaphorin-L, Sema L, CD108.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
SEMA7A produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 846 amino acids (45-648 a.a.) and having a molecular mass of 95.7kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
SEMA7A is expressed with an 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
SEMA7A (semaphorin-7A isoform 1) is a membrane-bound protein that belongs to the semaphorin family. It is attached to the cell surface through a glycosylphosphatidylinositol (GPI) anchor. SEMA7A plays a crucial role in cell signaling by promoting the formation of focal adhesion complexes and activating the protein kinase PTK2/FAK1, which in turn leads to the phosphorylation of MAPK3 and MAPK1. This signaling pathway is important for cell migration and immune responses. SEMA7A is involved in integrin-mediated signaling and regulates cell movement. Furthermore, it stimulates macrophages and monocytes to produce pro-inflammatory cytokines, contributing to the inflammatory process.
Recombinant SEMA7A protein, produced in Sf9 insect cells, is a single polypeptide chain with glycosylation. It consists of 846 amino acids (residues 45-648) and has a molecular weight of 95.7 kDa. However, on SDS-PAGE, it appears as a band between 70-100 kDa due to glycosylation. The protein includes a 242 amino acid hIgG-His tag at the C-terminus and is purified using proprietary chromatographic techniques.
The product is a sterile, colorless solution.
The SEMA7A protein solution is provided at a concentration of 0.25 mg/ml in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, adding a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing of the product should be avoided.
The purity of the SEMA7A protein is greater than 90%, as determined by SDS-PAGE analysis.
Semaphorin-7A, CDw108, JMH blood group antigen, John-Milton-Hargen human blood group Ag, Semaphorin-K1, Sema K1, Semaphorin-L, Sema L, CD108.
Sf9, Insect cells.
ADPQGHLRSG PRIFAVWKGH VGQDRVDFGQ TEPHTVLFHE PGSSSVWVGG RGKVYLFDFP EGKNASVRTV NIGSTKGSCL DKRDCENYIT LLERRSEGLL ACGTNARHPS CWNLVNGTVV PLGEMRGYAP FSPDENSLVL FEGDEVYSTI RKQEYNGKIP RFRRIRGESE LYTSDTVMQN PQFIKATIVH QDQAYDDKIY YFFREDNPDK NPEAPLNVSR VAQLCRGDQG GESSLSVSKW NTFLKAMLVC SDAATNKNFN RLQDVFLLPD PSGQWRDTRV YGVFSNPWNY SAVCVYSLGD IDKVFRTSSL KGYHSSLPNP RPGKCLPDQQ PIPTETFQVA DRHPEVAQRV EPMGPLKTPL FHSKYHYQKV AVHRMQASHG ETFHVLYLTT DRGTIHKVVE PGEQEHSFAF NIMEIQPFRR AAAIQTMSLD AERRKLYVSS QWEVSQVPLD LCEVYGGGCH GCLMSRDPYC GWDQGRCISI YSSERSVLQS INPAEPHKEC PNPKPDKAPL QKVSLAPNSR YYLSCPMESR HATYSWRHKE NVEQSCEPGH QSPNCILFIE NLTAQQYGHY FCEAQEGSYF REAQHWQLLP EDGIMAEHLL GHACALALEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.
Product Science Overview
Sema7A is an approximately 80 kDa membrane-anchored glycoprotein that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage . It is expressed in various adult tissues, including the spleen, colon, esophagus, heart, brain, spleen, testis, lung, ovary, and uterus . On human erythrocytes, it is recognized as the JMH blood group antigen .
Sema7A plays a crucial role in both the nervous and immune systems. In the nervous system, it is involved in axon guidance, cell differentiation, and neuron projection development . In the immune system, Sema7A acts as a potent immunomodulator, influencing the behavior of lymphoid and myeloid cells . It has been shown to modulate cellular immune responses, particularly in the context of autoimmune diseases like rheumatoid arthritis (RA) .
Research has highlighted the therapeutic potential of Sema7A in treating autoimmune diseases. For instance, elevated levels of soluble Sema7A (sSema7A) have been observed in the serum and synovial fluid of patients with RA, correlating with disease activity markers . Sema7A has been found to significantly increase the secretion of proinflammatory cytokines and activate T cells and monocytes . Blocking Sema7A with specific antibodies has shown promise in reducing inflammation and disease progression in experimental models of RA .
