SEMA3C Human

SEMA3C, also called Semaphorin 3C, belongs to the semaphorin family 3, which comprises eight classes based on their structure and phylogenetic relationships. Class 3 semaphorins play a crucial role following central nervous system injuries. SEMA3C is found in scar tissue and influences the behavior of neurons and other cell types in the injured area. It is present in all motor neurons, developing cardiac neural crest cells, and lung buds. SEMA3C interacts with Plexin-D1 and either Neuropilin 1 or Neuropilin 2 coreceptors to exert its effects. While primarily known for its repulsive guidance cues, SEMA3C can also act as a chemoattractant by activating integrins in certain cells.

Recombinant human SEMA3C, produced in HEK cells, is a single, glycosylated polypeptide chain. It encompasses amino acids 21 to 738, resulting in a 951 amino acid protein with a molecular weight of 107.2 kDa. The protein includes a 233 amino acid hIgG-Tag fused to its C-terminus. Purification is achieved using proprietary chromatographic methods.

A clear, sterile-filtered solution.

The SEMA3C solution has a concentration of 1 mg/ml and is prepared in a buffer containing 10% glycerol and Phosphate-Buffered Saline (pH 7.4).

For short-term storage (up to 4 weeks), the solution should be kept at 4°C. Long-term storage requires freezing at -20°C. Adding a carrier protein (HSA or BSA) at a concentration of 0.1% is recommended for extended storage. Repeated freezing and thawing should be avoided.

The purity of the protein is determined to be greater than 90% using SDS-PAGE analysis.

Semaphorin 3C ,Semaphorin-3C, Semaphorin-3C isoform2, SEMA3C, Semaphorin-E, SEMAE, Sema E, SemE, SEME, Semaphorin E

HEK293 Cells.

GSSQPQARVY LTFDELRETK TSEYFSLSHH PLDYRILLMD EDQDRIYVGS KDHILSLNIN NISQEALSVF WPASTIKVEE CKMAGKDPTH GCGNFVRVIQ TFNRTHLYVC GSGAFSPVCT YLNRGRRSED QVFMIDSKCE SGKGRCSFNP NVNTVSVMIN EELFSGMYID FMGTDAAIFR SLTKRNAVRT DQHNSKWLSE PMFVDAHVIP DGTDPNDAKV YFFFKEKLTD NNRSTKQIHS MIARICPNDT GGLRSLVNKW TTFLKARLVC SVTDEDGPET HFDELEDVFL LETDNPRTTL VYGIFTTSSS VFKGSAVCVY HLSDIQTVFN GPFAHKEGPN HQLISYQGRI PYPRPGTCPG GAFTPNMRTT KEFPDDVVTF IRNHPLMYNS IYPIHKRPLI VRIGTDYKYT KIAVDRVNAA DGRYHVLFLG TDRGTVQKVV VLPTNNSVSG ELILEELEVF KNHAPITTMK ISSKKQQLYV SSNEGVSQVS LHRCHIYGTA CADCCLARDP YCAWDGHSCS RFYPTGKRRS AAQDVRHGNP LTQCRGFNLK AYRNAAEIVQ YGVKNNTTFL ECAPKSPQAS IKWLLQKDKD AAKEVKLNER IIATSQGLLI RSVQGSDQGL YHCIATENSF KQTIAKINFK VLDSEMVAVV TDKWSPWTWA SSVRALPFHP KDIMGAFSHS EMQMINQYCK DTRQQHQQGD ESQKMRGDYG KLKALINSLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K