SDHAF1 Human
Succinate Dehydrogenase Complex Assembly Factor 1 Human Recombinant
Cat. No.
BT4399
Source
Escherichia Coli.
Synonyms
Succinate Dehydrogenase Complex Assembly Factor 1, LYR
Motif-Containing Protein 8, LYR Motif
Containing 8, SDH Assembly Factor 1, LYRM8, Succinate Dehydrogenase Assembly
Factor 1, Mitochondrial, Succinate dehydrogenase assembly factor 1,
mitochondrial.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
SDHAF1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 138 amino acids (1-115 a.a) and
having a molecular mass of 15.2kDa.
SDHAF1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
Succinate dehydrogenase assembly factor 1 (SDHAF1) is a protein belonging to the complex I LYR family. It plays a crucial role in the assembly of the succinate dehydrogenase complex (SDH), a component of complex II in the mitochondrial electron transport chain. SDHAF1 facilitates the biosynthesis of iron-sulfur centers within mitochondria, which are essential for the proper functioning of complex II.
Description
This product consists of recombinant human SDHAF1 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain with 138 amino acids (specifically, amino acids 1-115), resulting in a molecular weight of 15.2 kDa. The protein includes a 23-amino acid His-tag at the N-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation
The SDHAF1 protein is supplied in a solution with a concentration of 0.25 mg/ml. The solution is buffered to pH 7.4 using phosphate-buffered saline (PBS) and contains 30% glycerol, 2 mM dithiothreitol (DTT), and 0.1 mM phenylmethylsulfonyl fluoride (PMSF).
Stability
For short-term storage (up to 4 weeks), the product should be kept at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as albumin (HSA or BSA) at a concentration of 0.1% is advised. Repeated freezing and thawing of the product should be avoided.
Purity
Analysis by SDS-PAGE indicates that the purity of this product exceeds 85%.
Synonyms
Succinate Dehydrogenase Complex Assembly Factor 1, LYR
Motif-Containing Protein 8, LYR Motif
Containing 8, SDH Assembly Factor 1, LYRM8, Succinate Dehydrogenase Assembly
Factor 1, Mitochondrial, Succinate dehydrogenase assembly factor 1,
mitochondrial.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSRHSRL QRQVLSLYRD LLRAGRGKPG AEARVRAEFR QHAGLPRSDV
LRIEYLYRRG RRQLQLLRSG HATAMGAFVR PRAPTGEPGG VGSQPDDGDS PRNPHDSTGA PETRPDGR.
Product Science Overview
Structure and Function
SDHAF1 is a small protein that shields the SDHB subunit of the SDH complex from oxidative damage, thereby facilitating the proper assembly and function of the SDH complex . The human recombinant form of SDHAF1 is typically produced in E. coli and consists of a single, non-glycosylated polypeptide chain containing 138 amino acids, with a molecular mass of approximately 15.2 kDa . It is often fused to a His-tag at the N-terminus to aid in purification .
Role in Disease
Mutations in the SDHAF1 gene have been linked to various human diseases, including certain types of cancer and neurodegenerative disorders . Defective assembly of the SDH complex due to mutations in SDHAF1 can lead to impaired mitochondrial function and increased oxidative stress, contributing to the pathogenesis of these diseases .
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