SDF 1g Human

Stromal Cell-Derived Factor-1 gamma Human Recombinant (CXCL12)
Cat. No.
BT24031
Source
Escherichia Coli.
Synonyms
Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, CXCL12, SDF1, hSDF-1gamma, SDF-1g, TLSF, TPAR1, SCYB12.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SDF-1 gamma Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 99 amino acids and having a molecular mass of 11.6kDa.
The SDF-1g is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Stromal cell-derived factor-1 (SDF-1), also known as Chemokine (C-X-C motif) ligand 12 (CXCL12), is a chemokine that plays a crucial role in directing cell migration and coordinating cellular processes. Existing in two forms, SDF-1a/CXCL12a and SDF-1b/CXCL12b, this signaling molecule is highly chemotactic for lymphocytes, influencing their movement within the body. During development, SDF-1 is vital for guiding hematopoietic cells from the fetal liver to the bone marrow, highlighting its importance in immune system development. Mice lacking the CXCL12 gene exhibit lethality either before or shortly after birth, underscoring the critical role of this chemokine. Beyond its role in immune cell development, SDF-1 also impacts neuronal function by modulating the electrical activity of nerve cells. Widely expressed in various tissues such as the brain, thymus, heart, lungs, liver, kidneys, spleen, and bone marrow, SDF-1 exerts its effects by binding to its receptor, CXCR4, which was previously termed fusin. While this interaction was previously considered highly specific, recent findings suggest that SDF-1 may also engage with the CXCR7 receptor. The genes encoding both CXCL12 and CXCR4 exhibit a high degree of conservation between humans and mice, indicating their evolutionary significance. In humans, the CXCL12 gene resides on chromosome 10, while in both humans and mice, the sequences of CXCL12 and CXCR4 share remarkable similarity, with 99% and 90% identity, respectively.
Description
SDF-1 gamma Human Recombinant, a product of E.coli expression, is a single, non-glycosylated polypeptide chain composed of 99 amino acids. With a molecular weight of 11.6kDa, this purified protein is obtained through proprietary chromatographic techniques.
Physical Appearance
White, sterile-filtered powder obtained through lyophilization.
Formulation
SDF-1g protein undergoes lyophilization from a concentrated solution (0.2µm filtered) in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute lyophilized SDF-1g, it is recommended to dissolve it in sterile 18M-cm H₂O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized SDF-1g remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it desiccated at temperatures below -18°C. After reconstitution, SDF-1 gamma should be stored at 4°C for 2-7 days. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
Exceeds 96.0% purity as determined by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) and Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analyses.
Biological Activity
The biological activity, as measured by a chemotaxis bioassay utilizing PHA and rHuIL-2 activated human peripheral blood T-lymphocytes, falls within the concentration range of 30-100 ng/ml.
Synonyms
Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, CXCL12, SDF1, hSDF-1gamma, SDF-1g, TLSF, TPAR1, SCYB12.
Source
Escherichia Coli.
Amino Acid Sequence
GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN.

Product Science Overview

Introduction

Stromal Cell-Derived Factor-1 gamma (SDF-1γ), also known as CXCL12γ, is a member of the CXC chemokine family. Chemokines are small cytokines or signaling proteins secreted by cells, and they play a crucial role in immune responses by directing the migration of circulating leukocytes to sites of inflammation or injury.

Gene and Protein Structure

The CXCL12 gene is located on chromosome 10 in humans and produces several isoforms through alternative splicing . The gamma isoform, SDF-1γ, is a 12 kDa protein that binds heparin and is characterized by a typical chemokine-like fold with three antiparallel beta-strands . The protein is not glycosylated and contains four conserved cysteine residues that form two disulfide bonds, a hallmark of the CXC chemokine family .

Biological Functions

CXCL12γ functions as a ligand for the G-protein coupled receptor CXCR4. This interaction is pivotal in various physiological processes, including:

  • Embryogenesis: Directing the migration of hematopoietic cells from the fetal liver to the bone marrow.
  • Immune Surveillance: Attracting lymphocytes to sites of inflammation.
  • Tissue Homeostasis: Maintaining the microenvironment of various tissues.
  • Tumor Growth and Metastasis: CXCL12 signaling has been observed in several cancers, where it can promote tumor growth and metastasis .
Clinical Significance

The role of CXCL12γ in disease states is significant. It is upregulated in conditions characterized by tissue hypoxia, such as myocardial infarction and ischemic cardiomyopathy . Additionally, the CXCL12 gene contains single nucleotide polymorphisms (SNPs) associated with an increased risk of coronary artery disease .

Recombinant Human CXCL12γ

Recombinant human CXCL12γ is produced in E. coli and is used in various research applications. It is typically purified to a high degree of purity (>95%) and is used to study its biological activities, such as its ability to chemoattract cells expressing CXCR4 . The recombinant protein is available in both carrier-free and carrier-containing formulations, with the latter often containing bovine serum albumin (BSA) to enhance stability .

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