SDF2 Human, sf9

Stromal Cell-Derived Factor 2, Sf9 Human Recombinant
Cat. No.
BT24232
Source
Sf9, Baculovirus cells.
Synonyms

Stromal Cell Derived Factor 2, Stromal Cell-Derived Factor 2, SDF-2 

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

SDF2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 202 amino acids (19-211a.a.) and having a molecular mass of 22.3kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SDF2 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Stromal Cell-Derived Factor 2 (SDF2) is a secretory protein that shares partial similarity with the hydrophilic segments of yeast mannosyltransferases. SDF2 protein is ubiquitously expressed, and the corresponding gene exhibits significant conservation among mammals. SDF2 is a protein-coding gene with alternative splicing, resulting in both coding and non-coding variants.
Description
SDF2, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 202 amino acids (19-211a.a.) with a molecular mass of 22.3 kDa. On SDS-PAGE, the molecular size appears approximately between 18-28 kDa. SDF2 is expressed with a 9 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
SDF2 protein solution (0.25 mg/ml) is formulated in 50 mM Tris-HCl (pH 8.0), 10% glycerol, 0.1 M NaCl, 0.1 mM PMSF, and 0.5 mM EDTA.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 95.0% purity as determined by SDS-PAGE.
Synonyms

Stromal Cell Derived Factor 2, Stromal Cell-Derived Factor 2, SDF-2 

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

ADPSSLGVVT CGSVVKLLNT RHNVRLHSHD VRYGSGSGQQ SVTGVTSVDD SNSYWRIRGK SATVCERGTP IKCGQPIRLT HVNTGRNLHS HHFTSPLSGN QEVSAFGEEG EGDYLDDWTV LCNGPYWVRD GEVRFKHSST EVLLSVTGEQ YGRPISGQKE VHGMAQPSQN NYWKAMEGIF MKPSELLKAE AHHAELHHHH HH

Product Science Overview

Gene and Protein Structure

The SDF2 gene is a protein-coding gene that undergoes alternative splicing, resulting in both coding and non-coding variants . The protein produced by this gene is believed to be a secretory protein, which means it is synthesized in the cell and then secreted out to perform its functions .

Recombinant SDF2 Production

Recombinant SDF2 is produced in Sf9 Baculovirus cells. The recombinant protein is a single, glycosylated polypeptide chain containing 202 amino acids (19-211a.a.) and has a molecular mass of approximately 22.3 kDa . The molecular size on SDS-PAGE appears at approximately 18-28 kDa . This recombinant protein is expressed with a 9 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

Physical and Chemical Properties

The recombinant SDF2 protein is provided as a sterile filtered colorless solution. The formulation typically contains 50mM Tris-HCl (pH 8.0), 10% glycerol, 0.1M NaCl, 0.1mM PMSF, and 0.5mM EDTA . It is recommended to store the protein at 4°C if it will be used within 2-4 weeks, or at -20°C for longer periods. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised to avoid multiple freeze-thaw cycles .

Applications and Usage

SDF2 has various applications in laboratory research. It is used in studies related to cell signaling, protein interactions, and other cellular processes. The protein’s high purity (greater than 95.0% as determined by SDS-PAGE) makes it suitable for various biochemical assays and experiments .

Safety and Handling

ProSpec’s recombinant SDF2 is intended for laboratory research use only. It is not to be used as drugs, agricultural or pesticidal products, food additives, or household chemicals .

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