SCRN1 Human

Secernin 1 Human Recombinant
Cat. No.
BT14663
Source
Escherichia Coli.
Synonyms
Secernin-1, SES1, KIAA0193, Secernin 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SCRN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 437 amino acids (1-414 a.a) and having a molecular mass of 48.8kDa. SCRN1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Secernin 1 (SCRN1), a member of the peptidase C69 family, plays a role in regulating exocytosis within mast cells. It exhibits the ability to elevate both the extent of secretion and the sensitivity of mast cells to calcium-mediated stimulation.
Description
Recombinant human SCRN1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 437 amino acids (specifically, amino acids 1 to 414). Its molecular weight is 48.8 kDa. The protein is engineered with a 23-amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, filtered solution that appears colorless.
Formulation
The SCRN1 protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer containing phosphate-buffered saline (pH 7.4), 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 90.0% based on SDS-PAGE analysis.
Synonyms
Secernin-1, SES1, KIAA0193, Secernin 1.
Source
Escherichia Coli.
Amino Acid Sequence
GSSHHHHHH SSGLVPRGSH MGSMAAAPPS YCFVAFPPRA KDGLVVFGKN SARPRDEVQE VVYFSAADHE PESKVECTYI SIDQVPRTYA IMISRPAWLW GAEMGANEHG VCIANEAINT REPAAEIEAL LGMDLVRLGL ERGETAKEAL DVIVSLLEEH GQGGNYFEDA NSCHSFQSAY LIVDRDEAWV LETIGKYWAA EKVTEGVRCI CSQLSLTTKM DAEHPELRSY AQSQGWWTGE GEFNFSEVFS PVEDHLDCGA GKDSLEKQEE SITVQTMMNT LRDKASGVCI DSEFFLTTAS GVSVLPQNRS SPCIHYFTGT PDPSRSIFKP FIFVDDVKLV PKTQSPCFGD DDPAKKEPRF QEKPDRRHEL YKAHEWARAI IESDQEQGRK LRSTMLELEK QGLEAMEEIL TSSEPLDPAE VGDLFYDCVD TEIKFFK.

Product Science Overview

Introduction

Secernin 1, also known as SCRN1, is a protein that has garnered significant interest in the scientific community due to its various roles in cellular processes and disease mechanisms. This article delves into the background, structure, function, and significance of Secernin 1, particularly focusing on its recombinant form used in research and therapeutic applications.

Structure and Characteristics

Secernin 1 is a cytosolic protein with a molecular weight of approximately 50 kDa . The recombinant form of Secernin 1 is typically produced in Escherichia coli (E. coli) and includes an N-terminal His-tag for purification purposes . The amino acid sequence of the recombinant human Secernin 1 corresponds to residues 1-414 of the native protein .

Function and Mechanism

Secernin 1 was initially identified as a regulator of exocytosis in mast cells . It plays a crucial role in the secretion process by modulating the exocytosis of granules. This function is vital for various physiological processes, including immune responses and neurotransmitter release.

Role in Disease

Recent studies have highlighted the involvement of Secernin 1 in neurodegenerative diseases, particularly Alzheimer’s disease . Secernin 1 has been identified as a novel amyloid plaque-associated protein. Immunohistochemistry studies have shown that Secernin 1 is present in plaque-associated dystrophic neurites and co-localizes with neurofibrillary tangles (NFTs) in Alzheimer’s disease . This unique association with tau pathology suggests that Secernin 1 could serve as a potential therapeutic target and biomarker for distinguishing Alzheimer’s disease from other tauopathies .

Recombinant Secernin 1 in Research

The recombinant form of Secernin 1 is widely used in research to study its function and role in disease. The protein is produced in E. coli and purified using affinity chromatography techniques . This recombinant protein is essential for various assays, including SDS-PAGE and immunohistochemistry, to investigate its interactions and functions at the molecular level .

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