SCN3B Human, Sf9
Sf9, Baculovirus cells.
Greater than 95.0% as determined by SDS-PAGE.
Description
SCN3B Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 146 amino acids (23-159a.a.) and having a molecular mass of 16.8kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
SCN3B is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Sf9, Baculovirus cells.
ADPFPVCVEV PSETEAVQGN PMKLRCISCM KREEVEATTV VEWFYRPEGG KDFLIYEYRN GHQEVESPFQ GRLQWNGSKD LQDVSITVLN VTLNDSGLYT CNVSREFEFE AHRPFVKTTR LIPLRVTEEA GEDFTSVVSE HHHHHH.
Product Science Overview
Sodium channels are essential components in the physiology of excitable cells, such as neurons and muscle cells. These channels are responsible for the initiation and propagation of action potentials, which are critical for communication within the nervous system and for muscle contraction. The sodium channel voltage-gated, type III beta (SCN3B) is a specific subunit of these channels that plays a crucial role in modulating their function.
Voltage-gated sodium channels are complex transmembrane glycoproteins composed of a large alpha subunit and one or more regulatory beta subunits . The alpha subunit forms the ion conduction pore, while the beta subunits modulate the channel’s gating kinetics and other properties . The SCN3B gene encodes the beta-3 subunit of the sodium channel, which influences the inactivation kinetics of the sodium channel .
The beta-3 subunit is known to cause unique persistent sodium currents and inactivates the sodium channel opening more slowly than the beta-1 subunit . This modulation is crucial for the proper functioning of the sodium channels, affecting the generation and propagation of action potentials in neurons and muscle cells .
The SCN3B gene is located on chromosome 11 in humans . It has been identified to have two alternatively spliced variants, both encoding the same protein . The gene is expressed in various tissues, including the brain and heart, indicating its importance in both the nervous and cardiovascular systems .
Mutations in the SCN3B gene have been associated with several clinical conditions. Notably, it has been linked to Brugada Syndrome 7 and Familial Atrial Fibrillation . These conditions are characterized by abnormal heart rhythms, which can lead to severe cardiac events. The role of SCN3B in these conditions underscores the importance of proper sodium channel function in maintaining normal cardiac and neural activity.
The human recombinant SCN3B protein can be expressed in Sf9 cells, a type of insect cell line commonly used for protein production . This recombinant expression system allows for the production of large quantities of the protein for research purposes. The recombinant SCN3B protein is used in various studies to understand its function and role in disease, as well as to develop potential therapeutic interventions .
