SAT1 Human

Spermidine/Spermine N1-Acetyltransferase 1 Human Recombinant

Cat. No.

BT13409

Source

Escherichia Coli.

Synonyms

Diamine acetyltransferase 1, Spermidine/spermine N(1)-acetyltransferase 1, Putrescine acetyltransferase, Polyamine N-acetyltransferase 1, SSAT-1, SSAT, SAT1, SAT, DC21, KFSD, KFSDX.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

SAT1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids (1-171 a.a.) and having a molecular mass of 22.1kDa.
The SAT1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction

SAT-1, an enzyme critical for polyamine homeostasis, belongs to the acetyltransferase family and plays a crucial role in polyamine metabolism. As a rate-limiting enzyme, it catalyzes the acetylation of spermidine and spermine, thereby regulating their intracellular concentration and facilitating their transport out of cells. This regulatory function is vital as acetylated products are either excreted or oxidized by acetylpolyamine oxidase. Dysregulation of SAT1 activity can lead to various adverse effects, including pancreatic cell death, impaired regenerative tissue growth, behavioral abnormalities, keratosis follicularis spinulosa decalvans (KFSD), and hair loss. Notably, defects in the SAT1 gene are implicated in KFSD, a rare X-linked disorder characterized by skin and eye abnormalities. Affected individuals typically experience skin thickening, particularly in the neck, ears, extremities, palms, and soles, along with hair loss, eyelid thickening, and corneal degeneration. While most cases exhibit X-linked inheritance, KFSD presents with clinical and genetic heterogeneity.

Description

Recombinant human SAT1, engineered with a 20 amino acid His tag at the N-terminus, is produced in E. coli. This non-glycosylated polypeptide chain comprises 191 amino acids (1-171 a.a.) and exhibits a molecular weight of 22.1kDa. Purification is achieved using proprietary chromatographic methods.

Physical Appearance

A clear, colorless solution that has been sterilized by filtration.

Formulation

The SAT1 solution is prepared in a buffer containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.

Stability

For short-term storage (up to 2-4 weeks), store the entire vial at 4°C. For long-term storage, freezing at -20°C is recommended. To enhance stability during extended storage, adding a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freeze-thaw cycles.

Purity

SDS-PAGE analysis confirms a purity exceeding 95.0%.

Synonyms

Diamine acetyltransferase 1, Spermidine/spermine N(1)-acetyltransferase 1, Putrescine acetyltransferase, Polyamine N-acetyltransferase 1, SSAT-1, SSAT, SAT1, SAT, DC21, KFSD, KFSDX.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMATE E.

Product Science Overview

Introduction

Spermidine/Spermine N1-Acetyltransferase 1 (SAT1), also known as SSAT1, is a crucial enzyme in the polyamine metabolism pathway. This enzyme is responsible for the acetylation of polyamines, specifically spermidine and spermine, using acetyl-coenzyme A (CoA) as a substrate. The acetylation process alters the charge of these polyamines, facilitating their secretion or further oxidation by acetylpolyamine oxidase.

Structure and Function

SAT1 belongs to the acetyltransferase family and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. The enzyme’s primary function is to regulate the intracellular concentration of polyamines and their transport out of cells. Polyamines, including spermidine, spermine, and putrescine, are essential for various cellular processes such as DNA stabilization, protein synthesis, and cell proliferation.

Role in Cellular Homeostasis

SAT1 plays a critical role in maintaining polyamine homeostasis within mammalian cells. Under normal conditions, SAT1 is expressed at low levels. However, its expression, protein level, and activity can be significantly altered during tumorigenesis. These alterations can lead to cellular damage, including oxidative stress, cell cycle arrest, and changes in DNA dynamics and proliferation.

Clinical Significance

The expression of SAT1 has been reported to be altered in various cancers, and it has been correlated with tumor development and progression. Tumor grades and stages are often associated with the expression levels of SAT1. This enzyme can serve as a target for substrate binding, and its excreted metabolites may be used as novel cancer biomarkers. Additionally, polyamine analogs that increase SAT1 expression have shown potential in enhancing the cytotoxicity of chemotherapy to tumor cells.

Recombinant SAT1

Recombinant human SAT1 is produced using recombinant DNA technology, which involves inserting the human SAT1 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in various research applications, including studying the enzyme’s structure, function, and role in disease processes.

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SAT1 Human

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