SARS MERS, (18-751)
Sf9, Baculovirus cells.
Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERSCoV S1 P, Spike1 glycoprotein, S1 glycoprotein, S1, Spike S1 Subunit protein, S1 Subunit
Greater than 85.0% as determined by SDS-PAGE.
Description
SARS MERS Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 740 amino acids (18-751aa) and having a molecular mass of 82.0kDa.
SARS MERS is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) has been a concern since April 2012, with cases reported globally. Coronaviruses, responsible for illnesses ranging from the common cold to severe conditions like SARS (severe acute respiratory syndrome), are a family of viruses known for high mortality rates. MERS-CoV, a novel strain within this family, causes serious pneumonia characterized by sudden and severe respiratory complications, often leading to death. As of January 27th, 2015, the World Health Organization (WHO) has reported 956 cases and 351 deaths from MERS-CoV. A surge in new cases is anticipated. Similar to other coronaviruses, MERS-CoV possesses a large surface spike glycoprotein crucial for its structure. This protein, situated on the virion surface, facilitates binding and entry into host cells. The spike protein is divided into two domains: S1 and S2. S1 dictates cellular tropism and interaction with the target cell, while S2 manages membrane fusion. The C-terminal of the S1 domain, housing a receptor binding domain, presents a promising target for vaccine development and serves as a valuable antigen for diagnostic purposes.
SARS MERS Recombinant, produced using Sf9 insect cells and Baculovirus expression system, is a single, glycosylated polypeptide chain. This protein consists of 740 amino acids (spanning from position 18 to 751), resulting in a molecular weight of 82.0kDa. For purification and detection purposes, a 6 amino acid His-tag is fused to the C-terminus of the SARS MERS protein. Purification is achieved through proprietary chromatographic techniques.
SARS MERS is supplied in a solution with a concentration of 0.25mg/ml. This solution is formulated to contain 10% glycerol and Phosphate-Buffered Saline, maintaining a pH of 7.4.
For short-term storage (up to 4 weeks), keep the SARS MERS vial refrigerated at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein, such as HSA or BSA at a concentration of 0.1%, is advised for extended storage. Avoid repeated freezing and thawing of the product to maintain its integrity.
The purity of SARS MERS is determined using SDS-PAGE analysis, indicating a purity level exceeding 85%.
Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERSCoV S1 P, Spike1 glycoprotein, S1 glycoprotein, S1, Spike S1 Subunit protein, S1 Subunit
Sf9, Baculovirus cells.
YVDVGPDSVK SACIEVDIQQ TFFDKTWPRP IDVSKADGII YPQGRTYSNI TITYQGLFPY QGDHGDMYVY SAGHATGTTP QKLFVANYSQ DVKQFANGFV VRIGAAANST GTVIISPSTS ATIRKIYPAF MLGSSVGNFS DGKMGRFFNH TLVLLPDGCG TLLRAFYCIL EPRSGNHCPA GNSYTSFATY HTPATDCSDG NYNRNASLNS FKEYFNLRNC TFMYTYNITE DEILEWFGIT QTAQGVHLFS SRYVDLYGGN MFQFATLPVY DTIKYYSIIP HSIRSIQSDR KAWAAFYVYK LQPLTFLLDF SVDGYIRRAI DCGFNDLSQL HCSYESFDVE SGVYSVSSFE AKPSGSVVEQ AEGVECDFSP LLSGTPPQVY NFKRLVFTNC NYNLTKLLSL FSVNDFTCSQ ISPAAIASNC YSSLILDYFS YPLSMKSDLS VSSAGPISQF NYKQSFSNPT CLILATVPHN LTTITKPLKY SYINKCSRLL SDDRTEVPQL VNANQYSPCV SIVPSTVWED GDYYRKQLSP LEGGGWLVAS GSTVAMTEQL QMGFGITVQY GTDTNSVCPK LEFANDTKIA SQLGNCVEYS LYGVSGRGVF QNCTAVGVRQ QRFVYDAYQN LVGYYSDDGN YYCLRACVSV PVSVIYDKET KTHATLFGSV ACEHISSTMS QYSRSTRSML KRRDSTYGPL QTPVGCVLGL VNSSLFVEDC KLPLGQSLCA LPDTPSTLTP RSVRHHHHHH
Product Science Overview
The SARS MERS Spike S1 (18-751 a.a.) Recombinant protein is a crucial component in the study of coronaviruses, particularly SARS-CoV (Severe Acute Respiratory Syndrome Coronavirus) and MERS-CoV (Middle East Respiratory Syndrome Coronavirus). These viruses belong to the Betacoronavirus genus and have caused significant outbreaks in the past two decades. The spike (S) protein of these viruses plays a vital role in their ability to infect host cells.
The spike protein is a transmembrane glycoprotein that forms homotrimers protruding from the viral surface. It is composed of two subunits: S1 and S2. The S1 subunit is responsible for binding to the host cell receptor, while the S2 subunit facilitates membrane fusion. The S1 subunit contains the receptor-binding domain (RBD), which is critical for the virus’s attachment to the host cell.
The recombinant form of the spike S1 protein, specifically the 18-751 amino acid (a.a.) segment, is engineered to mimic the natural protein’s structure and function. This segment includes the RBD and other essential regions necessary for studying the virus’s interaction with host cells.
The recombinant spike S1 protein is typically produced using mammalian cell expression systems, such as HEK293 cells. These systems ensure proper folding and post-translational modifications, which are crucial for the protein’s functionality. The production process involves transfecting the cells with a plasmid containing the gene encoding the spike S1 protein. After expression, the protein is purified using affinity chromatography techniques, often involving a His-tag or other affinity tags for easy isolation.
The SARS MERS Spike S1 (18-751 a.a.) Recombinant protein has several applications in research and diagnostics:
- Vaccine Development: The spike protein is a primary target for vaccine development. Recombinant spike proteins are used to generate immune responses in animal models and humans, aiding in the creation of effective vaccines.
- Antibody Production: The recombinant protein is used to produce monoclonal and polyclonal antibodies. These antibodies are essential for detecting the virus in diagnostic assays and for therapeutic purposes.
- Diagnostic Assays: The spike S1 protein is used in various diagnostic assays, such as ELISA (Enzyme-Linked Immunosorbent Assay) and lateral flow immunoassays, to detect antibodies against the virus in patient samples.
- Structural Studies: Researchers use the recombinant protein to study the structure of the spike protein and its interaction with host cell receptors. These studies provide insights into the mechanisms of viral entry and potential therapeutic targets.
