Sf9, Baculovirus cells.
Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERSCoV RBD, MERS RBD, receptor binding domain, RBD, Spike RBD protein, Spike glycoprotein, S glycoprotein, E2, Peplomer protein
Greater than 90.0% as determined by SDS-PAGE.
SARS MERS RBD Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 258 amino acids (358-606 aa) and having a molecular mass of 28.2kDa. SARS MERS RBD is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERSCoV RBD, MERS RBD, receptor binding domain, RBD, Spike RBD protein, Spike glycoprotein, S glycoprotein, E2, Peplomer protein
Sf9, Baculovirus cells.
ADPSGVYSVS SFEAKPSGSV VEQAEGVECD FSPLLSGTPP QVYNFKRLVF TNCNYNLTKL LSLFSVNDFT CSQISPAAIA SNCYSSLILD YFSYPLSMKS DLSVSSAGPI SQFNYKQSFS NPTCLILATV PHNLTTITKP LKYSYINKCS RLLSDDRTEV PQLVNANQYS PCVSIVPSTV WEDGDYYRKQ LSPLEGGGWL VASGSTVAMT EQLQMGFGIT VQYGTDTNSV CPKLEFANDT KIASQLGNCV EYHHHHHH.
The spike (S) protein of coronaviruses, including SARS-CoV (Severe Acute Respiratory Syndrome Coronavirus) and MERS-CoV (Middle East Respiratory Syndrome Coronavirus), plays a crucial role in the virus’s ability to infect host cells. The receptor-binding domain (RBD) within the spike protein is responsible for binding to the host cell receptors, facilitating viral entry. Recombinant forms of these RBDs are used in research and vaccine development due to their ability to elicit immune responses.
The spike protein is a trimeric class I fusion protein that protrudes from the viral surface. It consists of two subunits: S1 and S2. The S1 subunit contains the RBD, which is responsible for recognizing and binding to the host cell receptor. For SARS-CoV, the RBD binds to the angiotensin-converting enzyme 2 (ACE2) receptor, while for MERS-CoV, it binds to the dipeptidyl peptidase 4 (DPP4) receptor .
Recombinant RBDs are produced using recombinant DNA technology. This involves inserting the gene encoding the RBD into an expression vector, which is then introduced into a host cell line (e.g., HEK293 cells). The host cells express the RBD protein, which can be harvested and purified for use in various applications .
The use of recombinant RBDs has significantly advanced our understanding of coronavirus biology and facilitated the development of vaccines and therapeutics. By targeting the RBD, researchers can develop strategies to block viral entry and prevent infection. Additionally, recombinant RBDs provide a safe and effective way to study highly pathogenic viruses without the need for live virus .