SAP18 Human

Sin3A-Associated Protein 18kDa Human Recombinant
Cat. No.
BT14196
Source
Escherichia Coli.
Synonyms
Histone deacetylase complex subunit SAP18, Sin3-associated polypeptide 18 kDa, Sin3-associated polypeptide p18, Cell growth-inhibiting protein 38, 2HOR0202, SAP18, GIG38.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SAP18 Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 173 amino acids (20- 172 a.a.) and having a molecular mass of 19.7 kDa.
The SAP18 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
SAP18, a crucial component of the histone deacetylase complex, plays a vital role in regulating eukaryotic gene expression. It directly interacts with SIN3, amplifying SIN3-mediated transcriptional repression upon promoter binding. SAP18 is essential for the targeted recruitment of the HDAC complex by various transcription factors, such as Gli, GAGA, and Bicoid. Histone acetylation is a critical process in regulating eukaryotic gene expression, and the acetylation and deacetylation of histones are facilitated by multisubunit complexes. SAP18 is an integral part of the histone deacetylase complex, which comprises SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. Moreover, SAP18 is a constituent of the splicing-dependent multiprotein EJC (exon junction complex) positioned at splice junctions on mRNAs.
Description
Recombinant Human SAP18, fused with a 20 amino acid His tag at the N-terminus, is produced in E. coli. This single, non-glycosylated polypeptide chain consists of 173 amino acids (20-172 a.a.) and has a molecular weight of 19.7 kDa. The purification of SAP18 is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The SAP18 solution is formulated in 20mM Tris-HCl buffer with a pH of 8.0, 0.1M NaCl, 30% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Histone deacetylase complex subunit SAP18, Sin3-associated polypeptide 18 kDa, Sin3-associated polypeptide p18, Cell growth-inhibiting protein 38, 2HOR0202, SAP18, GIG38.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY.

Product Science Overview

Introduction

Sin3A-Associated Protein 18kDa (SAP18) is a crucial component of the histone deacetylase (HDAC) complex, which plays a significant role in the regulation of eukaryotic gene expression. This protein is known for its interaction with Sin3A, a transcriptional repressor, and its involvement in various cellular processes.

Classification and Structure

SAP18 is classified as a part of the Sin3/HDAC corepressor complex, which is highly conserved from yeast to humans. The core components of this complex include Sin3, SAP30, SAP18, HDAC1, HDAC2, SDS3, RBBP4, and RBBP7 . The protein encoded by the SAP18 gene directly interacts with Sin3A and enhances Sin3A-mediated transcriptional repression when tethered to the promoter .

Biological Properties and Functions

SAP18 is involved in the regulation of gene expression through its role in the HDAC complex. Histone acetylation and deacetylation are key processes in the regulation of eukaryotic gene expression, and SAP18 plays a pivotal role in these processes. The protein enhances Sin3A-mediated transcriptional repression, which is crucial for maintaining proper gene expression levels .

Mode of Action

The mode of action of SAP18 involves its interaction with Sin3A and other components of the HDAC complex. SAP18 enhances the recruitment of Sin3A to regulatory elements of target genes, thereby increasing the transcriptional repression mediated by Sin3A. This interaction is essential for the regulation of various genes involved in lipid metabolism and other cellular processes .

Regulatory Mechanisms

SAP18 is regulated at multiple levels, including transcriptional and post-translational modifications. The expression of SAP18 can be modulated by various factors, and its activity is influenced by its interaction with other components of the HDAC complex. Additionally, SAP18 has been shown to play a role in the regulation of microsomal triglyceride transfer protein (MTTP) expression, which is important for lipid metabolism .

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