SAMSN1 Human
SAM Domain SH3 Domain and Nuclear Localization Signal 1 Human Recombinant
Cat. No.
BT14096
Source
Escherichia Coli.
Synonyms
HACS1, NASH1, SASH2, SH3D6B, SLy2, SAM domain-containing protein SAMSN-1, Hematopoietic adaptor containing SH3 and SAM domains 1, Nash1, SAM domain, SH3 domain and nuclear localization signals protein 1, SH3-SAM adaptor protein, SAMSN1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SAMSN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 396 amino acids (1-373a.a) and having a molecular mass of 44.1kDa.
SAMSN1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SAMSN1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
SAM Domain SH3 Domain and Nuclear Localization Signal 1 (SAMSN1), a member of the adaptor and scaffold protein family, possesses both SH3 and SAM (sterile alpha motif) domains. This protein plays a crucial role in negatively regulating B-cell activation and subsequently suppressing cell proliferation. Its expression is induced by IL-4 in activated B cells, and it is predominantly found in dendritic cells. SAMSN1's function closely resembles that of other adaptor proteins, effectively linking signaling molecules within signal transduction cascades.
Description
Recombinant SAMSN1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 396 amino acids (residues 1-373). With a molecular weight of 44.1 kDa, it features a 23 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The SAMSN1 protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 30% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of SAMSN1 is determined to be greater than 80.0% based on SDS-PAGE analysis.
Synonyms
HACS1, NASH1, SASH2, SH3D6B, SLy2, SAM domain-containing protein SAMSN-1, Hematopoietic adaptor containing SH3 and SAM domains 1, Nash1, SAM domain, SH3 domain and nuclear localization signals protein 1, SH3-SAM adaptor protein, SAMSN1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLKRKPS NVSEKEKHQK PKRSSSFGNF DRFRNNSLSK PDDSTEAHEG DPTNGSGEQS KTSNNGGGLG KKMRAISWTM KKKVGKKYIK ALSEEKDEED GENAHPYRNS DPVIGTHTEK VSLKASDSMD SLYSGQSSSS GITSCSDGTS NRDSFRLDDD GPYSGPFCGR ARVHTDFTPS PYDTDSLKIK KGDIIDIICK TPMGMWTGML NNKVGNFKFI YVDVISEEEA APKKIKANRR SNSKKSKTLQ EFLERIHLQE YTSTLLLNGY ETLEDLKDIK ESHLIELNIE NPDDRRRLLS AAENFLEEEI IQEQENEPEP LSLSSDISLN KSQLDDCPRD SGCYISSGNS DNGKEDLESE NLSDMVHKII ITEPSD.
Product Science Overview
Historical Background
The SAMSN1 gene is located on human chromosome 21q11.2, a region frequently disrupted by translocation events in hematopoietic malignancies . The full-length cDNA of human SAMSN1 was first identified and cloned in 2001 from myeloma cells and human cord blood-derived mast cells . The gene was named HACS1 and Nash1 by different research groups .
Protein Structure
SAMSN1 encodes a 441 amino acid protein containing several key domains:
- Sterile Alpha Motif (SAM): Located toward the C-terminal end, this domain is involved in protein-protein interactions.
- Src Homology 3 (SH3) Domain: Located in the middle half of the protein, this domain shares 39% homology with the well-characterized adaptor protein Crk .
- Nuclear Localization Signal (NLS): There are three predicted consensus nuclear localization signals .
- Tyrosine Kinase Phosphorylation Motif: Predicted at amino acids 221–228 .
Expression and Localization
SAMSN1 is expressed in various hematopoietic tissues, particularly in B cells, macrophages, mast cells, and dendritic cells . It is also expressed in the adult heart, kidney, placenta, lung, bone marrow, peripheral blood, and immune tissues, including lymph nodes, spleen, and thymus . In embryonic mice, SAMSN1 is highly expressed in blood vessels, brain, future spinal cord, dorsal root ganglia, otocyst, eye, limb, heart, surface ectoderm, and bronchial arch .
Function
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