S100A8 Mouse

S100 Calcium Binding Protein A8 Mouse Recombinant
Cat. No.
BT743
Source
Escherichia Coli.
Synonyms
Calgranulin A, MRP8, CAGA, CGLA, CFAG, Protein S100-A8, S100 calcium-binding protein A8, Migration inhibitory factor-related protein 8, MRP-8, p8, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A, S100A8, MIF, NIF, L1Ag, CP-10, MA387, 60B8AG.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

S100A8 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (1-89a.a) and having a molecular mass of 12.4kDa.
S100A8 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100A8, a member of the S100 protein family, plays a crucial role in immune responses and cellular processes. This protein, containing two EF-hand calcium-binding motifs, is involved in various functions, including the inhibition of casein kinase, cytokine activity, and regulation of cell cycle progression and differentiation. S100A8 is implicated in several biological processes, including: - Antimicrobial activity against bacteria and fungi, contributing to host defense against pathogens. - Regulation of inflammatory responses, acting as a pro-inflammatory mediator in acute and chronic inflammation. - Promotion of phagocyte migration and infiltration of granulocytes at sites of injury. - Modulation of gene transcription, particularly those under the control of NF-kappa-B. Alterations in S100A8 expression have been linked to diseases such as cystic fibrosis and endotoxic shock.
Description
Recombinant S100A8 from mouse has been produced in an E. coli expression system. This non-glycosylated polypeptide chain consists of 109 amino acids, including a 20 amino acid His-tag at the N-terminus, resulting in a molecular weight of 12.4 kDa. Purification is achieved through proprietary chromatographic techniques, ensuring high purity of the recombinant protein.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
The S100A8 protein is supplied in a solution at a concentration of 1 mg/ml. The formulation buffer consists of 20 mM Tris-HCl (pH 8.0), 30% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is advisable. It is important to avoid repeated freezing and thawing of the product.
Purity
The purity of S100A8 is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms
Calgranulin A, MRP8, CAGA, CGLA, CFAG, Protein S100-A8, S100 calcium-binding protein A8, Migration inhibitory factor-related protein 8, MRP-8, p8, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A, S100A8, MIF, NIF, L1Ag, CP-10, MA387, 60B8AG.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPSELEKALS NLIDVYHNYS NIQGNHHALY KNDFKKMVTT ECPQFVQNIN IENLFRELDI NSDNAINFEE FLAMVIKVGV ASHKDSHKE

Product Science Overview

Introduction

S100 Calcium Binding Protein A8, also known as S100A8, is a member of the S100 family of proteins. These proteins are characterized by their ability to bind calcium ions through EF-hand motifs, which are helix-loop-helix structural domains . S100A8 is predominantly expressed in cells of the myeloid lineage, such as neutrophils and monocytes .

Structure and Expression

S100A8 is a small protein with a molecular mass of approximately 10.3 kDa . It contains two EF-hand calcium-binding motifs, which are crucial for its function . The protein is mainly localized in the cytoplasm but can translocate to the plasma membrane or be released into the extracellular environment upon cellular activation .

Function

S100A8 plays a significant role in the regulation of inflammatory processes and the immune response . It can induce neutrophil chemotaxis and adhesion, which are essential for the immune system’s response to infection and injury . S100A8 often forms a heterodimer with S100A9, another member of the S100 family, to create a complex known as calprotectin . This complex has a wide range of intra- and extracellular functions, including the regulation of leukocyte adhesion and migration, promotion of cytokine and chemokine production, and antimicrobial activity .

Clinical Relevance

Altered expression of S100A8 is associated with various diseases, including cystic fibrosis and psoriasis . In cystic fibrosis, the protein’s expression is significantly increased, which may contribute to the chronic inflammation observed in patients . Additionally, S100A8 has been implicated in the pathogenesis of other inflammatory diseases and certain cancers .

Recombinant S100A8

Recombinant S100A8 (Mouse) is produced using Escherichia coli expression systems . The recombinant protein is used in various research applications, including studies on inflammation, immune response, and cancer . It is available in different formulations and concentrations, with high purity levels confirmed by SDS-PAGE analysis .

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