S100A5 Human
Description
S100A5 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 112 amino acids (1-92 a.a.) and having a molecular mass of 12.9kDa. The S100A5 is purified by proprietary chromatographic techniques.
Product Specs
Recombinant human S100A5, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a 20 amino acid His tag attached to its N-terminus. It consists of 112 amino acids, including the 92 amino acids of S100A5 (residues 1-92), resulting in a molecular weight of 12.9 kDa. The purification process for S100A5 involves proprietary chromatographic techniques.
For short-term storage (up to 4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to store the solution at -20°C. To enhance stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. It is crucial to minimize freeze-thaw cycles to maintain product integrity.
Product Science Overview
S100A5 contains two EF-hand calcium-binding motifs, which are responsible for its high affinity for calcium ions. Interestingly, S100A5 has a Ca2+ affinity that is 20- to 100-fold higher than other S100 proteins studied under identical conditions . Additionally, S100A5 can bind zinc (Zn2+) and copper (Cu2+) ions, with Cu2+ binding strongly enough to impair the binding of Ca2+ .
Due to its high affinity for calcium and its ability to bind other metal ions, S100A5 is of interest in various research areas, including neurobiology and cancer research. Its role in cell cycle regulation and differentiation makes it a valuable target for studying cellular processes and potential therapeutic interventions .
