S100A4 Mouse

S100 Calcium Binding Protein A4 Mouse Recombinant
Cat. No.
BT30095
Source
Escherichia Coli.
Synonyms
Protein S100-A4, S100 calcium-binding protein A4, Metastasin, Protein Mts1, Placental calcium-binding protein, Calvasculin, S100A4, CAPL, MTS1, 42A, 18A2, FSP1, P9KA, PEL98.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

S100A4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 121 amino acids (1-101 a.a) and having a molecular mass of 13.9kDa.
S100A4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100A4, a member of the S100 protein family, plays a crucial role in various cellular processes, including cell cycle progression, differentiation, motility, invasion, and tubulin polymerization. This protein, containing two EF-hand calcium-binding motifs, is structurally similar to other calcium-binding proteins like calmodulin and troponin C. S100 proteins, including S100A4, are found in various cells, including antigen-presenting cells like Langerhans cells and interdigitating reticulum cells. Notably, S100A4 is implicated in tumor metastasis, with chromosomal rearrangements and altered expression of its gene being linked to the process.
Description
Recombinant S100A4 Mouse protein, expressed in E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 13.9kDa. It consists of 121 amino acids, including a 20 amino acid His-tag at the N-terminus, covering amino acids 1-101 of the S100A4 sequence. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The S100A4 protein solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.1M NaCl, and 2mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. The addition of a carrier protein, such as HSA or BSA (0.1%), is advised for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the S100A4 protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Protein S100-A4, S100 calcium-binding protein A4, Metastasin, Protein Mts1, Placental calcium-binding protein, Calvasculin, S100A4, CAPL, MTS1, 42A, 18A2, FSP1, P9KA, PEL98.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MARPLEEALD VIVSTFHKYS GKEGDKFKLN KTELKELLTR ELPSFLGKRT DEAAFQKVMS NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGCPDKEPRK K.

Product Science Overview

Introduction

S100 Calcium Binding Protein A4 (S100A4), also known as metastasin, is a member of the S100 family of proteins. These proteins are characterized by their ability to bind calcium ions through EF-hand motifs, which are helix-loop-helix structural domains . S100A4 is encoded by the S100A4 gene and is involved in various cellular processes, including motility, invasion, and tubulin polymerization .

Gene and Protein Structure

The S100A4 gene is located on chromosome 3 in mice and chromosome 1 in humans . The protein itself contains two EF-hand calcium-binding motifs, which are crucial for its function . These motifs allow S100A4 to interact with other proteins and participate in calcium-dependent signaling pathways .

Biological Functions

S100A4 is involved in several important biological processes:

  • Cell Motility and Invasion: S100A4 increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain IIA (MYH9) .
  • Angiogenesis: It supports tumorigenesis by stimulating angiogenesis, often in synergy with vascular endothelial growth factor (VEGF) .
  • Cell Differentiation and Apoptosis: S100A4 plays a role in cell differentiation and apoptosis, contributing to the regulation of cell cycle progression .
Clinical Significance

S100A4 has been implicated in various pathological conditions, particularly in cancer. Its overexpression is associated with tumor metastasis and poor prognosis in several types of cancer . Additionally, S100A4 is involved in inflammation and has been linked to various inflammation-associated diseases .

Recombinant S100A4

Recombinant S100A4 protein, particularly from mouse sources, is often used in research to study its functions and interactions. The recombinant protein is typically expressed in E. coli and purified using conventional chromatography techniques . This allows researchers to investigate the protein’s role in cellular processes and its potential as a therapeutic target.

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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