S100A1 Human

S100 Calcium Binding Protein A1 Human Recombinant
Cat. No.
BT29140
Source
Escherichia Coli.
Synonyms
Protein S100-A1, S100 calcium-binding protein A1, S-100 protein alpha subunit, S-100 protein alpha chain, S100A1, S100A, S100, S100-alpha, S100-A1.
Appearance
White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

S100A1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids which include a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 11.66 kDa.
S100A1 Human Recombinant is purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100A1, a member of the S100 calcium-binding protein family, possesses an EF-hand motif for Ca+2 binding. This protein plays a crucial role in activating sarcoplasmic calcium release and regulating intermediate filament polymerization. Its functions encompass stimulating Ca2+-induced Ca2+ release, inhibiting microtubule assembly, and suppressing protein kinase C-mediated phosphorylation. Notably, reduced S100A1 expression has been linked to cardiomyopathies. S100 proteins are commonly found in the cytoplasm or nucleus of various cells. The S100 gene family, comprising at least 13 members, is clustered on chromosome 1q21.
Description
Recombinant human S100A1, expressed in E. coli, is a non-glycosylated polypeptide chain with a single chain. It consists of 103 amino acids, including a 10 amino acid His tag at the N-terminus, resulting in a molecular weight of 11.66 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Lyophilized powder, white in color.
Formulation
The S100A1 protein was lyophilized at a concentration of 0.5 mg/ml from a 0.4 μm filtered solution containing 20 mM Tris pH 7.5 and 50 mM NaCl.
Solubility
To prepare a working solution, add deionized water to achieve a concentration of approximately 0.5 mg/ml. Allow the lyophilized pellet to dissolve completely. Please note that this product is not sterile. Before using it in cell culture, ensure to filter the solution through an appropriate sterile filter.
Stability
Lyophilized S100A1 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated state below -18°C. After reconstitution, S100A1 can be stored at 4°C for 2-7 days. For extended storage, store below -18°C. To enhance stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
The purity of S100A1 is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Protein S100-A1, S100 calcium-binding protein A1, S-100 protein alpha subunit, S-100 protein alpha chain, S100A1, S100A, S100, S100-alpha, S100-A1.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS GSELETAMET LINVFHAHSG KEGDKYKLSK KELKELLQTE LSGFLDAQKD VDAVDKVMKE LDENGDGEVD FQEYVVLVAA LTVACNNFFW ENS.

Product Science Overview

Introduction

S100 Calcium Binding Protein A1, also known as S100A1, is a member of the S100 family of proteins. These proteins are characterized by their ability to bind calcium ions through EF-hand motifs, which are helix-loop-helix structural domains . S100A1 is highly expressed in cardiac and skeletal muscle tissues, as well as in the brain .

Structure

S100A1 contains four EF-hand calcium-binding motifs in its dimerized form . It can exist as either a heterodimer or a homodimer, with the homodimer being formed through hydrophobic interactions between specific helices . The protein’s structure allows it to undergo conformational changes upon binding calcium ions, which is crucial for its function .

Function

S100A1 plays a significant role in various biological processes, including calcium homeostasis, chondrocyte biology, and cardiomyocyte regulation . In cardiomyocytes, S100A1 regulates a network controlling sarcoplasmic reticulum calcium cycling and mitochondrial function . It interacts with several key proteins, such as ryanodine receptors, sarcoplasmic reticulum calcium-ATPase, and mitochondrial F1-ATPase .

Clinical Significance

The expression of S100A1 has been implicated in several diseases. Reduced expression of this protein is associated with cardiomyopathies, which are diseases of the heart muscle that can lead to heart failure . Additionally, S100A1 has shown promise as a candidate for gene therapy to treat post-myocardial infarction cardiac tissue .

Recombinant S100A1

Human recombinant S100A1 is produced using recombinant DNA technology, which involves inserting the gene encoding S100A1 into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the protein for research and therapeutic purposes .

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S100A1 Mouse
S100 Calcium Binding Protein A1 Mouse Recombinant
Cat. No.
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Source
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