S100A16 Human
S100 Calcium Binding Protein A16 Human Recombinant
Cat. No.
BT29778
Source
Escherichia Coli.
Synonyms
Protein S100-A16, Aging-associated gene 13 protein, Protein S100-F, S100 calcium-binding protein A16, S100A16, S100F, AAG13, DT1P1A7, MGC17528.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
S100A16 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 123 amino acids (1-103 a.a.) and having a molecular mass of 13.9kDa.
S100A16 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
S100A16 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
S100A16, a member of the S100 protein family, possesses calcium-binding EF-hand motifs. These proteins demonstrate cell and tissue specificity and participate in various intracellular and extracellular processes by interacting with specific target proteins. Notably, S100A16 expression is exclusive to astrocytes. It accumulates within nucleoli and, upon calcium stimulation, relocates to the cytoplasm. Furthermore, S100A16 physically interacts with the tumor suppressor protein p53, a known inhibitor of adipogenesis, as demonstrated through immunoprecipitation analysis.
Description
Produced in E. coli, S100A16 is a single, non-glycosylated polypeptide chain comprising 123 amino acids (specifically, amino acids 1 through 103). It has a molecular weight of 13.9 kDa. The protein includes a 20 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
The provided S100A16 solution (1 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 40% glycerol, and 0.2 M NaCl.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For longer periods, storage at -20°C in a frozen state is recommended. To ensure stability during long-term storage, adding a carrier protein like 0.1% HSA or BSA is advisable. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of S100A16 is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Protein S100-A16, Aging-associated gene 13 protein, Protein S100-F, S100 calcium-binding protein A16, S100A16, S100F, AAG13, DT1P1A7, MGC17528.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSDCYTELEK AVIVLVENFY KYVSKYSLVK NKISKSSFRE MLQKELNHML SDTGNRKAAD KLIQNLDANH DGRISFDEYW TLIGGITGPI AKLIHEQEQQ SSS.
Product Science Overview
Structure and Characteristics
S100A16 is a small acidic protein consisting of 103 amino acids, with a molecular weight of approximately 12 kDa . It contains two EF-hand motifs, which are helix-loop-helix structural domains responsible for calcium binding. The N-terminal EF-hand of S100A16 is unique as it comprises 15 amino acids instead of the typical 14 and lacks the conserved glutamate residue at the final position, which may impair its calcium-binding capability .
Expression and Localization
S100A16 is widely expressed in various human tissues and is highly conserved among mammals . It is located in several cellular components, including the cytosol, extracellular space, and nucleolus . The protein is involved in the differentiation of adipocytes and has been shown to increase proliferation and enhance adipogenesis in preadipocytes .
Biological Functions
S100A16 plays a significant role in several biological processes:
- Calcium Binding: It binds one calcium ion per monomer, which is essential for its function .
- Adipogenesis: Overexpression of S100A16 in preadipocytes promotes their proliferation and enhances adipogenesis .
- Cancer: Abnormal expression of S100A16 has been implicated in the progression of various cancers, including breast and prostate cancer . It has also been shown to inhibit the proliferation of oral cancer and acute lymphoblastic leukemia tumor cells .
Recombinant S100A16
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