S100A15 Mouse

S100 Calcium Binding Protein A15 Mouse Recombinant
Cat. No.
BT29689
Source
E.coli.
Synonyms
Protein S100-A7A, S100 calcium-binding protein A15A, S100 calcium-binding protein A7A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

S100A15 Mouse Recombinant produced in E. coli is a single polypeptide chain containing 132 amino acids (1-108) and having a molecular mass of 15.0 kDa.
S100A15 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mouse S100A15 is a protein that consists of 104 amino acids. It has a molecular weight of 12,870 Dalton and contains two EF-hand calcium binding sites. S100A15 expression is increased in cultured keratinocytes that are stimulated to differentiate by calcium or phorbol esters. It is found in the skin and keratinocytes.
Description
Recombinant Mouse S100A15 is produced in E. coli. It is a single polypeptide chain containing 132 amino acids (amino acids 1-108) with a molecular mass of 15.0 kDa. It is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless, and filtered solution.
Formulation
The S100A15 solution has a concentration of 0.25mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 150mM NaCl, and 20% glycerol.
Stability
If the entire vial will be used within 2-4 weeks, store at 4°C. For longer storage periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
Purity is greater than 85% as determined by SDS-PAGE.
Synonyms
Protein S100-A7A, S100 calcium-binding protein A15A, S100 calcium-binding protein A7A.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMPDTPV EDSLFQIIHC FHHYAAREGD KETLSLEELK ALLLDSVPRF MDTLGRRQPY YITELFRAAD KNKDNQICFD EFLYILGKLV KDYHLQFHRQ LCAHYCTEHS LY.

Product Science Overview

Introduction

S100 Calcium Binding Protein A15 (S100A15) is a member of the S100 protein family, which is characterized by the presence of two EF-hand calcium-binding motifs. These proteins are involved in a variety of intracellular and extracellular functions, including regulation of protein phosphorylation, enzyme activities, cell growth and differentiation, and the inflammatory response.

Structure and Function

S100A15, like other S100 proteins, has a structure that allows it to bind calcium ions, which is crucial for its function. The binding of calcium induces a conformational change in the protein, enabling it to interact with target proteins and influence various cellular processes. S100A15 is known to play a role in the regulation of inflammatory responses and has been implicated in various skin conditions.

Recombinant S100A15

Recombinant S100A15 refers to the protein that has been produced through recombinant DNA technology. This involves inserting the gene encoding S100A15 into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. The recombinant protein is then purified for use in research and therapeutic applications.

Applications in Research

Recombinant S100A15 is used extensively in research to study its role in inflammation and skin diseases. It is also used to investigate the mechanisms by which S100 proteins interact with other cellular components and to develop potential therapeutic interventions for conditions involving dysregulated inflammation.

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