S100A13 Human

S100 Calcium Binding Protein A13 Human Recombinant

Cat. No.

BT29537

Source

Escherichia Coli.

Synonyms

Protein S100-A13, S100 calcium-binding protein A13, S100A13.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

S100A13 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 118 amino acids (1-98) and having a molecular mass of 13.6 kDa.
The S100A13 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction

S100A13 is a member of the S100 family of proteins, which are characterized by their two EF-hand calcium-binding motifs. S100 proteins are found in a variety of cells, where they are located in the cytoplasm and/or nucleus. They play regulatory roles in numerous cellular processes, including cell cycle progression and differentiation. S100A13 is widely expressed in various tissues, with particularly high levels of expression in the thyroid gland. Within smooth muscle cells, S100A13 is found in the nucleus and stress fibers, often co-expressed with other S100 family members. This suggests that S100A13 may have multiple functions in signal transduction.

Description

Recombinant human S100A13, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 118 amino acids (residues 1-98). It has a molecular weight of 13.6 kDa. The S100A13 protein includes a 20 amino acid His-Tag fused at the N-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.

Physical Appearance

A clear, sterile-filtered solution.

Formulation

The S100A13 protein is supplied at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 50 mM NaCl, and 20% glycerol.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. Avoid subjecting the product to repeated freeze-thaw cycles.

Purity

The purity of the S100A13 protein is greater than 95.0%, as determined by SDS-PAGE analysis.

Synonyms

Protein S100-A13, S100 calcium-binding protein A13, S100A13.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAAEPLTELE ESIETVVTTF FTFARQEGRK DSLSVNEFKE LVTQQLPHLL KDVGSLDEKM KSLDVNQDSE LKFNEYWRLI GELAKEIRKK KDLKIRKK

Product Science Overview

Introduction

S100 Calcium Binding Protein A13, also known as S100A13, is a member of the S100 family of proteins. These proteins are characterized by their ability to bind calcium ions through EF-hand motifs. S100A13 is a small, generally acidic protein that plays a crucial role in various cellular processes, including cell cycle progression and differentiation .

Structure and Function

S100A13 is composed of 98 amino acids and has a molecular weight of approximately 11.3 kilodaltons. The protein contains two EF-hand calcium-binding motifs, which allow it to bind calcium ions with differing affinities. Additionally, S100A13 has a basic residue-rich sequence at its C-terminus.

One of the unique features of S100A13 is its ability to bind copper ions. This binding is essential for the protein’s role in the copper-dependent export of fibroblast growth factor 1 (FGF-1) and interleukin 1 alpha (IL-1α) from the cell in response to stress conditions such as heat shock, anoxia, and starvation. The binding of copper ions does not interfere with the protein’s ability to bind calcium ions.

Biological Role

S100A13 is involved in the regulation of several cellular processes. It plays a significant role in the export of proteins that lack a signal peptide and are secreted via an alternative pathway. The protein is required for the copper-dependent stress-induced export of IL-1α and FGF-1. S100A13 also binds to lipid vesicles containing phosphatidylserine, but not to those containing phosphatidylcholine.

Clinical Significance

S100A13 has been proposed as a marker for angiogenesis in tumors and the endometrium due to its role in the stress-induced export of FGF-1. Additionally, it has been found to promote neurite outgrowth from rat cortical embryonic neurons. The protein’s involvement in these processes makes it a potential target for therapeutic interventions in various diseases, including cancer and neurodegenerative disorders.

Recombinant S100A13

Recombinant human S100A13 is produced using Escherichia coli expression systems and is available for research purposes. The recombinant protein is typically purified to a high degree of purity (>95%) and is suitable for various applications, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry (MS).

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S100A13 Human

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