RYBP Human

RING1 & YY1 Binding Protein Human Recombinant
Cat. No.
BT13947
Source
E.coli.
Synonyms
RING1 and YY1 binding protein, Death effector domain-associated factor, Apoptin-associating protein 1, YY1 and E4TF1-associated factor 1, ring1 interactor RYBP, DED-associated factor, APAP-1, DEDAF, YEAF1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RYBP Human Recombinant produced in E. coli is a single polypeptide chain containing 252 amino acids (1-228) and having a molecular mass of 27.4 kDa.
RYBP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RING1- and YY1-binding protein (RYBP) is a member of the polycomb group (PcG) family of proteins. It interacts with MDM2, inhibiting MDM2-mediated ubiquitination of p53. This interaction stabilizes p53, increasing its activity and leading to cell cycle arrest. RYBP is involved in the p53 response to DNA damage. It interacts with multiple proteins including RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. By preventing TP53 ubiquitination and degradation, RYBP plays a role in regulating the transcription of TP53 target genes. It may also act as a transcriptional repressor of E4TF1 and potentially bind to DNA to promote apoptosis.
Description
Recombinant human RYBP, produced in E. coli, is a single polypeptide chain with a molecular weight of 27.4 kDa. It consists of 252 amino acids, including amino acids 1-228 of the RYBP protein and a 24 amino acid His-tag fused at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RYBP solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the RYBP solution should be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of RYBP is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
RING1 and YY1 binding protein, Death effector domain-associated factor, Apoptin-associating protein 1, YY1 and E4TF1-associated factor 1, ring1 interactor RYBP, DED-associated factor, APAP-1, DEDAF, YEAF1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF

Product Science Overview

Structure and Function

RYBP is known for its ability to bind to both RING1 and YY1 proteins. It is involved in the monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a key modification that leads to chromatin compaction and gene silencing . This modification is crucial for the regulation of gene expression, particularly in the context of developmental genes such as Hox genes .

Biological Roles

  1. Gene Regulation: RYBP is a core component of the PRC1-like complex, which is involved in the repression of gene transcription. It helps in recruiting the complex to specific genomic sites, thereby facilitating the ubiquitination of histone H2A .

  2. Chromatin Remodeling: By participating in the modification of histones, RYBP plays a significant role in chromatin remodeling, which is essential for the heritable changes in gene expression .

  3. Apoptosis Regulation: RYBP has been shown to inhibit the ubiquitination and subsequent degradation of TP53, a crucial tumor suppressor protein. This regulation is vital for the transcription of TP53 target genes, which are involved in cell cycle control and apoptosis .

  4. Developmental Processes: RYBP is implicated in various developmental processes, including neural development and spermatogenesis. It is expressed in murine spermatocytes and plays a role in meiosis, although it is not essential for spermatogenesis .

Clinical Implications

RYBP has been studied for its potential role in cancer biology. It is believed to act as a tumor suppressor by down-regulating genes involved in tumor growth and metastasis, such as SRRM3 in breast cancer . Additionally, its role in regulating TP53 makes it a significant player in the cellular response to DNA damage and stress.

Research and Applications

The recombinant form of RYBP is used in various research applications to study its function and interactions. It is particularly valuable in understanding the mechanisms of gene regulation and the role of PcG proteins in development and disease.

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