RUNX3 Human
Runt-Related Transcription Factor 3 Human Recombinant
Cat. No.
BT13726
Source
Escherichia Coli.
Synonyms
AML2, CBFA3, PEBP2aC, PEBP2A3, FLJ34510, MGC16070, RUNX3, Oncogene AML-2, Runt-related transcription factor 3, Core-binding factor subunit alpha-3, Acute myeloid leukemia 2 protein, Polyomavirus enhancer-binding protein 2 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RUNX3 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (53-186 a.a.) and having a molecular mass of 17.1 kDa. The RUNX3 is fused to 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
RUNX3, a member of the RUNX family, plays a crucial role in regulating gene expression for cellular differentiation and cell cycle progression. It forms a heterodimer with its beta subunit, binding to the DNA sequence 5'-PYGPYGGT-3' found in enhancers and promoters. This complex can either activate or suppress transcription, interacting with other transcription factors. RUNX3's involvement in gastric cancer makes it a potential tumor suppressor diagnosis target.
Description
Recombinant Human RUNX3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 155 amino acids (53-186 a.a.). With a molecular weight of 17.1 kDa, it features a 21 amino acid His-Tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
RUNX3 Human solution (0.5mg/ml) in 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), refrigerate at 4°C. For longer periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
AML2, CBFA3, PEBP2aC, PEBP2A3, FLJ34510, MGC16070, RUNX3, Oncogene AML-2, Runt-related transcription factor 3, Core-binding factor subunit alpha-3, Acute myeloid leukemia 2 protein, Polyomavirus enhancer-binding protein 2 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MRSMVDVLAD HAGELVRTDS PNFLCSVLPS HWRCNKTLPV AFKVVALGDV PDGTVVTVMA GNDENYSAEL RNASAVMKNQ VARFNDLRFV GRSGRGKSFT LTITVFTNPT QVATYHRAIK VTVDGPREPR RHRQK.
Product Science Overview
Structure and Function
RUNX3 is characterized by the presence of a Runt domain (amino acids 54-186), which is essential for DNA binding. Additionally, it contains a proline/serine/threonine-rich region (amino acids 191-415) that is involved in the transcriptional activation of target genes . RUNX3 forms dimers with the core-binding factor beta (CBF-beta), which enhances its DNA-binding affinity and stability .
Biological Roles
RUNX3 is involved in several critical biological processes:
- Growth Control of Gastric Epithelium: RUNX3 is necessary for the regulation of cell growth in the gastric epithelium. It acts as a tumor suppressor in gastric cancer by inhibiting the proliferation of gastric epithelial cells .
- Neurogenesis of Dorsal Root Ganglia: RUNX3 plays a vital role in the development of the nervous system, particularly in the neurogenesis of dorsal root ganglia .
- T Cell Differentiation: RUNX3 is essential for the differentiation of T cells, which are crucial components of the immune system .
Clinical Significance
RUNX3 has been implicated in various cancers and other diseases:
- Tumor Suppression: RUNX3 acts as a tumor suppressor in several types of cancers, including gastric cancer, colon cancer, and lung cancer. Its expression is often silenced in tumors due to promoter hypermethylation, loss of heterozygosity, or histone modifications .
- Acute Myeloid Leukemia (AML): RUNX3 is also known as AML2 due to its involvement in acute myeloid leukemia. It plays a role in the regulation of hematopoiesis and is frequently mutated in AML .
Recombinant RUNX3
Recombinant RUNX3 is produced using various expression systems, such as E. coli. The recombinant protein is typically tagged with a His-tag for purification purposes and is available in lyophilized form for research use . It is used in various research applications to study its function and role in different biological processes and diseases.
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