RTN4R Human
Reticulon 4 Receptor Human Recombinant
Cat. No.
BT13575
Source
Sf9, Baculovirus cells.
Synonyms
Reticulon 4 Receptor, Nogo-66 Receptor, Nogo Receptor,
NOGOR, NGR , Reticulon-4 Receptor, UNQ330/PRO526.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
RTN4R produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 429 amino acids (27-447 a.a.) and
having a molecular mass of 46.3kDa (Molecular size on SDS-PAGE will appear at
approximately 40-57 kDa). RTN4R is expressed with a 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Reticulon 4 Receptor, also known as RTN4R, functions as a receptor for RTN4, OMG, and MAG. This glycosylphosphoinositol (GPI)-anchored protein belongs to the Nogo receptor family and is primarily found on neurons and their axons. RTN4R plays a crucial role in regulating axonal regeneration and plasticity within the adult central nervous system, making it a promising drug target for treating various neurological conditions. These conditions include spinal cord injury, central nervous system lesions, stroke, and Alzheimer's disease.
Description
Produced in Sf9 Baculovirus cells, RTN4R is a single, glycosylated polypeptide chain consisting of 429 amino acids (27-447 a.a.). With a molecular mass of 46.3 kDa, it appears at approximately 40-57 kDa on SDS-PAGE analysis. This protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The RTN4R protein solution is provided at a concentration of 0.5 mg/ml and is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for extended storage periods. Avoid repeated freezing and thawing cycles.
Purity
The purity of RTN4R is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Reticulon 4 Receptor, Nogo-66 Receptor, Nogo Receptor,
NOGOR, NGR , Reticulon-4 Receptor, UNQ330/PRO526.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
CPGACVCYNE PKVTTSCPQQ GLQAVPVGIP AASQRIFLHG NRISHVPAAS
FRACRNLTIL WLHSNVLARI DAAAFTGLAL LEQLDLSDNA QLRSVDPATF HGLGRLHTLH LDRCGLQELG
PGLFRGLAAL QYLYLQDNAL QALPDDTFRD LGNLTHLFLH GNRISSVPER AFRGLHSLDR LLLHQNRVAH VHPHAFRDLG RLMTLYLFAN NLSALPTEAL APLRALQYLR
LNDNPWVCDC RARPLWAWLQ KFRGSSSEVP CSLPQRLAGR DLKRLAANDL QGCAVATGPY HPIWTGRATD
EEPLGLPKCC QPDAADKASV LEPGRPASAG NALKGRVPPG DSPPGNGSGP RHINDSPFGT LPGSAEPPLT AVRPEGSEPP GFPTSGPRRR PGCSRKNRTR SHCRLGQAGS
GGGGTGDSEG SLEHHHHHH.
Product Science Overview
Introduction
Reticulon 4 Receptor (RTN4R), also known as Nogo Receptor, is a protein encoded by the RTN4R gene in humans. This receptor is primarily involved in the inhibition of neurite outgrowth and plays a crucial role in regulating axonal regeneration and plasticity in the adult central nervous system . The receptor is known to interact with several ligands, including Reticulon 4 (Nogo), oligodendrocyte myelin glycoprotein (OMG), and myelin-associated glycoprotein (MAG) .
Preparation Methods
The human recombinant Reticulon 4 Receptor is typically produced using recombinant DNA technology. The process involves the following steps:
- Gene Cloning: The RTN4R gene is cloned into an appropriate expression vector.
- Transformation: The vector is introduced into a host cell, commonly E. coli or mammalian cells, through a process called transformation.
- Expression: The host cells are cultured under conditions that promote the expression of the RTN4R protein.
- Purification: The expressed protein is purified using techniques such as affinity chromatography, which ensures the isolation of the recombinant protein with high purity.
Chemical Reactions Analysis
The Reticulon 4 Receptor is involved in several biochemical pathways and interactions:
- Axonal Growth Inhibition: RTN4R mediates axonal growth inhibition through its interaction with Nogo, OMG, and MAG . This interaction triggers intracellular signaling cascades via the coreceptor NGFR, leading to the activation of Rho and subsequent reorganization of the actin cytoskeleton .
- Neuronal Plasticity: RTN4R plays a role in regulating neuronal plasticity, which is essential for the formation and maintenance of synapses during brain development .
- Apoptosis Protection: The receptor protects motoneurons against apoptosis, likely through its interaction with MAG .
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