RTN4IP1 Human

Reticulon 4 Interacting Protein 1 Human Recombinant

Cat. No.

BT13496

Source

E.coli.

Synonyms

Reticulon-4-interacting protein 1 mitochondrial, NOGO-interacting mitochondrial protein, RTN4IP1, NIMP.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

RTN4IP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 379 amino acids (41-396 a.a.) and having a molecular mass of 41.4kDa.
RTN4IP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

RTN4IP1, a mitochondrial protein, uniquely interacts with reticulon 4, a known inhibitor of spinal cord injury regeneration. This interaction may provide valuable insights into how reticulon proteins inhibit neurite growth.

Description

Recombinantly produced in E. coli, RTN4IP1 Human is a non-glycosylated polypeptide chain consisting of 379 amino acids (41-396 a.a.). With a molecular weight of 41.4 kDa, it features a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

A clear, sterile-filtered solution.

Formulation

This RTN4IP1 protein solution (1 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 30% glycerol, 0.1 M NaCl, and 1 mM DTT.

Stability

For optimal storage, keep at 4°C for use within 2-4 weeks. For longer periods, store frozen at -20°C. Consider adding a carrier protein (0.1% HSA or BSA) for extended storage. Avoid repeated freeze-thaw cycles.

Purity

SDS-PAGE analysis confirms purity exceeding 95%.

Synonyms

Reticulon-4-interacting protein 1 mitochondrial, NOGO-interacting mitochondrial protein, RTN4IP1, NIMP.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSTVMPAWV IDKYGKNEVL RFTQNMMMPI IHYPNEVIVK VHAASVNPID VNMRSGYGAT ALNMKRDPLH VKIKGEEFPL TLGRDVSGVV MECGLDVKYF KPGDEVWAAV PPWKQGTLSE FVVVSGNEVS HKPKSLTHTQ AASLPYVALT AWSAINKVGG LNDKNCTGKR VLILGASGGV GTFAIQVMKA WDAHVTAVCS QDASELVRKL GADDVIDYKS GSVEEQLKSL KPFDFILDNV GGSTETWAPD FLKKWSGATY VTLVTPFLLN MDRLGIADGM LQTGVTVGSK ALKHFWKGVH YRWAFFMASG PCLDDIAELV DAGKIRPVIE QTFPFSKVPE AFLKVERGHA RGKTVINVV.

Product Science Overview

Gene and Protein Structure

The RTN4IP1 gene is located on chromosome 6q21 and encodes a protein that belongs to the medium-chain dehydrogenases/reductase (MDR) family. This family of enzymes is characterized by a conserved Rossmann-fold motif, which is essential for binding NAD(P)H cofactors. The RTN4IP1 protein itself is composed of two domains: a C-terminal coenzyme binding domain and an N-terminal substrate binding domain.

Function and Mechanism

RTN4IP1 plays a crucial role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, which is essential for the development of the inner retina and optic nerve. It interacts with Reticulon 4 (RTN4), also known as NOGO, a protein that inhibits neurite outgrowth and regeneration following spinal cord injury. This interaction is significant because it helps to understand the mechanisms behind neuroregeneration and the inhibition thereof.

Clinical Significance

Mutations in the RTN4IP1 gene have been linked to several disorders, including optic atrophy 10, which can occur with or without ataxia, cognitive disability, and seizures. These mutations disrupt the normal function of the protein, leading to impaired neuronal development and function.

Research and Applications

The recombinant form of RTN4IP1 is used in various research applications to study its structure and function. Structural studies have revealed that RTN4IP1 forms a dimer and binds NADPH, although its exact enzymatic function remains to be fully elucidated. Understanding the structure and function of RTN4IP1 can provide insights into potential therapeutic targets for neurodegenerative diseases and conditions involving impaired neuroregeneration.

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RTN4IP1 Human

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