RTCA Human

RNA 3'-Terminal Phosphate Cyclase Human Recombinant
Cat. No.
BT13445
Source
Escherichia Coli.
Synonyms
RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1, RNA cyclase, RNA-3'-phosphate cyclase, RNA terminal phosphate cyclase domain-containing protein 1, RTC domain-containing protein 1, RTCA, RPC1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RTCA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 389 amino acids (1-366a.a) and having a molecular mass of 41.7kDa.
RTCA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RNA 3'-Terminal Phosphate Cyclase (RTCA) is a member of the RNA 3'-phosphate cyclase family. This protein plays a role in RNA metabolism by catalyzing the conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester in a reaction that requires ATP. RTCA is involved in specific stages of cellular RNA processing.
Description
Recombinant human RTCA protein was expressed in E. coli and purified to a single, non-glycosylated polypeptide chain. It encompasses amino acids 1-366 and has a molecular weight of 41.7 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus. Purification was achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered.
Formulation
The RTCA protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freezing and thawing of the product.
Purity
The purity of the RTCA protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1, RNA cyclase, RNA-3'-phosphate cyclase, RNA terminal phosphate cyclase domain-containing protein 1, RTC domain-containing protein 1, RTCA, RPC1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL.

Product Science Overview

Structure and Mechanism

The RNA 3’-Terminal Phosphate Cyclase operates through a multi-step mechanism :

  1. Adenylylation: The enzyme first forms a covalent intermediate with AMP (adenosine monophosphate) in the presence of ATP.
  2. Cyclization: The enzyme-AMP complex then interacts with the RNA substrate, converting the 3’-phosphate group into a 2’,3’-cyclic phosphodiester.
  3. Release: The cyclic phosphate is released, completing the reaction.

This process is essential for the maintenance of cyclic ends in tRNA splicing intermediates and the cyclization of the 3’ end of U6 snRNA .

Physiological Function

While the exact physiological role of RNA 3’-Terminal Phosphate Cyclase is not fully understood, it is believed to be involved in several critical RNA processing events :

  • tRNA Splicing: The enzyme may help maintain cyclic ends in tRNA splicing intermediates, which are crucial for proper tRNA maturation.
  • snRNA Processing: It could also play a role in the cyclization of the 3’ end of U6 snRNA, a component of the spliceosome involved in pre-mRNA splicing.
Conservation and Expression

RNA 3’-Terminal Phosphate Cyclase is highly conserved across different domains of life, including Eucarya, Bacteria, and Archaea . This conservation suggests that the enzyme performs an essential function in RNA metabolism. The enzyme is expressed in various mammalian tissues and cell lines, indicating its widespread importance .

Research and Applications

The human recombinant form of RNA 3’-Terminal Phosphate Cyclase has been extensively studied to understand its structure, function, and potential applications . Research has shown that the enzyme is nuclear and has a diffuse nucleoplasmic localization . This localization is consistent with its role in RNA processing events that occur in the nucleus.

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