RSU1 Human

Ras Suppressor Protein 1 Human Recombinant
Cat. No.
BT13378
Source
Escherichia Coli.
Synonyms
Ras Suppressor Protein 1, Rsu-1, RSP-1, Ras Suppressor Protein 1 Variant 1, Ras Suppressor Protein 1 Variant 2, Ras Suppressor Protein 1 Variant 3, RSP1, RSU1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RSU1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 300 amino acids (1-277) and having a molecular mass of 33.9 kDa.
RSU1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RSU1, a protein found in mouse and human cells, plays a role in the ras signal transduction pathway. This protein is involved in growth inhibition and nerve-growth factor-induced differentiation. Initially isolated in mice due to its ability to inhibit v-Ras transformation, RSU1 potentially regulates the Ras signal transduction pathway and can suppress v-Ras transformation in laboratory settings.
Description
Recombinantly produced in E. coli, RSU1 Human is a single, non-glycosylated polypeptide chain. It consists of 300 amino acids (specifically, residues 1-277) and has a molecular weight of 33.9 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of RSU1, and proprietary chromatographic techniques are employed.
Physical Appearance
A clear, sterile solution without any color.
Formulation
The RSU1 solution is provided at a concentration of 1 mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep the vial refrigerated at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. To further enhance stability during extended storage, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the protein is determined by SDS-PAGE and is confirmed to be greater than 90.0%.
Synonyms
Ras Suppressor Protein 1, Rsu-1, RSP-1, Ras Suppressor Protein 1 Variant 1, Ras Suppressor Protein 1 Variant 2, Ras Suppressor Protein 1 Variant 3, RSP1, RSU1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSKSLKK LVEESREKNQ PEVDMSDRGI SNMLDVNGLF TLSHITQLVL SHNKLTMVPP NIAELKNLEV LNFFNNQIEE LPTQISSLQK LKHLNLGMNR LNTLPRGFGS LPALEVLDLT YNNLSENSLP GNFFYLTTLR ALYLSDNDFE ILPPDIGKLT KLQILSLRDN DLISLPKEIG ELTQLKELHI QGNRLTVLPP ELGNLDLTGQ KQVFKAENNP WVTPIADQFQ LGVSHVFEYI RSETYKYLYG RHMQANPEPP KKNNDKSKKI SRKPLAAKNR.

Product Science Overview

Introduction

Ras Suppressor Protein 1 (RSU1) is a protein that plays a significant role in the Ras signal transduction pathway, which is crucial for various cellular processes including growth inhibition and differentiation. Initially identified for its ability to inhibit v-Ras transformation, RSU1 has been extensively studied in both mouse and human cell lines .

Discovery and Function

RSU1 was discovered as a suppressor of Ras-induced transformation, meaning it can inhibit the oncogenic activity of the Ras protein. Ras proteins are small GTPases that play a key role in cell proliferation, differentiation, and survival. Mutations in Ras genes are common in various cancers, making the study of RSU1 particularly important .

RSU1 localizes to cell-extracellular matrix adhesions, which are critical for cell migration and invasion. This localization suggests that RSU1 may play a role in regulating metastasis-related cellular processes .

Structural Insights

Recent studies have provided detailed insights into the structure of RSU1 and its interaction with other proteins. RSU1 interacts with PINCH1, a component of the integrin-linked kinase (ILK)/PINCH/Parvin (IPP) complex, which is essential for focal adhesion formation and signaling. The interaction between RSU1 and PINCH1 negatively regulates F-actin bundling, thereby affecting cell adhesion and migration .

Role in Cancer

RSU1’s role in cancer has been a subject of significant research. Elevated expression of RSU1 has been observed in various cancer types, including breast, liver, and brain cancers. Its role in regulating metastasis-related processes, such as cell invasion and migration, is still being explored. However, in vitro studies have shown that RSU1 can impair stress fiber formation and cell spreading, indicating its potential as a therapeutic target .

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