RSPO3 Human
Description
Recombinant Human R-Spondin-3 produced in HEK293 cells is a polypeptide chain starting at amino acid Gln at position 22 to amino acid Val at position 201, fused to an FC, 6 x His-tag at C-terminus, containing a total of 498 amino acids and having a Mw of 47.9 kDa. The protein migrates at 61kDa on SDS-PAGE.
RSPO3 is a truncated protein that lacks amino acid Gln at position 201 to amino acid H at position 272 and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
RSPO3 is characterized by the presence of two Furin-like repeats and one thrombospondin type-1 (TSP-1) domain . These structural features are crucial for its function in signal transduction via receptor tyrosine kinases . RSPO3 enhances the activity of Wnt proteins such as Wnt-1, Wnt-3a, and Wnt-7a, and antagonizes the activity of DKK-1, a known Wnt pathway inhibitor .
RSPO3 is widely expressed in various tissues and plays a pivotal role in several physiological processes. It supports adult stem cell proliferation and is involved in the regulation of tissue-specific processes such as bone formation, skeletal muscle development, pancreatic β-cell proliferation, and intestinal stem cell maintenance . Additionally, RSPO3 has been implicated in cancer biology due to its ability to modulate the Wnt signaling pathway .
Recombinant human RSPO3 is produced using mammalian cell expression systems, such as Chinese Hamster Ovary (CHO) cells . The recombinant protein is typically purified to high levels of purity (>95%) using techniques like SDS-PAGE and quantitative densitometry . The endotoxin levels are kept below 1.0 EU per 1 μg of protein to ensure its suitability for biological applications .
Recombinant RSPO3 is used extensively in research to study its effects on Wnt signaling and its potential therapeutic applications. It is particularly valuable in regenerative medicine, where it is used to promote stem cell proliferation and tissue regeneration . RSPO3 is also utilized in the development of organoid cultures, especially for intestinal and kidney tissues .
Recombinant RSPO3 is typically lyophilized from a filtered solution and can be reconstituted in PBS for use . It is shipped at ambient temperature and should be stored at -20 to -70°C to maintain its stability. Once reconstituted, it should be stored under sterile conditions at 2 to 8°C for short-term use or at -20 to -70°C for long-term storage .
