RSG1 Human

REM2 and RAB-Like Small GTPase 1 Human Recombinant

Cat. No.

BT13288

Source

Escherichia Coli.

Synonyms

REM2- and Rab-like small GTPase 1, RSG1, C1orf89, RP4-733M16.4.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

RSG1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-258 a.a) and having a molecular mass of 30.9kDa.
RSG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

REM2 and RAB-Like Small GTPase 1 (RSG1), also known as Rab GTPase-like protein 1, is a protein involved in intracellular transport. It likely functions in vesicle trafficking, specifically in the fusion of vesicles with target membranes. RSG1 plays a crucial role in building cilia, hair-like structures on cells, by controlling the movement of essential proteins to the base and tips of cilia. This protein may also be a downstream component of the planar cell polarity pathway, which helps establish organized cell arrangements within tissues. Furthermore, RSG1 participates in the process of exocytosis, where cells release substances by fusing vesicles with the cell membrane.

Description

This recombinant RSG1 protein, derived from humans, is produced in E. coli bacteria. It is a single polypeptide chain that lacks glycosylation and consists of 281 amino acids, specifically amino acids 1 to 258 of the full-length protein. With a molecular weight of 30.9 kDa, this protein includes a 23 amino acid His-tag attached to its N-terminus, facilitating purification through specialized chromatographic methods.

Physical Appearance

Clear and colorless solution that has been sterilized by filtration.

Formulation

The RSG1 protein is provided in a solution at a concentration of 0.25 mg/ml. This solution is buffered with 20mM Tris-HCl at a pH of 8.5 and also contains 0.2M NaCl, 50% glycerol, and 2mM DTT.

Stability

For short-term storage (up to four weeks), keep the solution refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure the protein's stability during long-term storage, consider adding a carrier protein like HSA or BSA at a concentration of 0.1%. Repeated freezing and thawing of the solution should be avoided.

Purity

Determined by SDS-PAGE analysis, the purity of this RSG1 protein is greater than 85%.

Synonyms

REM2- and Rab-like small GTPase 1, RSG1, C1orf89, RP4-733M16.4.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMARPPVP GSVVVPNWHE SAEGKEYLAC ILRKNRRRVF GLLERPVLLP PVSIDTASYK IFVSGKSGVG KTALVAKLAG LEVPVVHHET TGIQTTVVFW PAKLQASSRV VMFRFEFWDC GESALKKFDH MLLACMENTD AFLFLFSFTD RASFEDLPGQ LARIAGEAPG VVRMVIGSKF DQYMHTDVPE RDLTAFRQAW ELPLLRVKSV PGRRLADGRT LDGRAGLADV AHILNGLAEQ LWHQDQVAAG LLPNPPESAP E.

Product Science Overview

RAB-Like Small GTPase 1

RAB-Like Small GTPase 1 is part of the Rab family of small GTPases, which are key regulators of intracellular membrane trafficking. These proteins are involved in various steps of vesicle transport, including vesicle formation, movement along actin and tubulin networks, and membrane fusion.

Structure and Function

Rab proteins possess a GTPase fold, consisting of a six-stranded beta sheet flanked by five alpha helices. They switch between an inactive GDP-bound form and an active GTP-bound form, regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Rab proteins are anchored to membranes via prenyl groups on their C-terminus, allowing them to interact with various effector proteins.

Biological Significance

Rab GTPases play a critical role in maintaining cellular homeostasis by regulating membrane trafficking processes. Mutations in Rab genes can lead to a wide range of genetic diseases, including neurodegenerative disorders like Parkinson’s and Alzheimer’s diseases, as well as various cancers. Rab1, a well-studied member of this family, is involved in the secretory pathway, Golgi complex maintenance, and macroautophagy regulation.

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RSG1 Human

Description

Product Specs

Product Science Overview

Structure and Function

Biological Significance

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