RRAS2 Human

The Related RAS Viral (r-ras) Oncogene Homolog 2, also known as RRAS2, is a member of the Ras-like GTPase family. This protein is involved in the regulation of cell proliferation, differentiation, and survival. It is a plasma membrane-associated GTP-binding protein with intrinsic GTPase activity .

RRAS2 is a single, non-glycosylated polypeptide chain consisting of 221 amino acids and has a molecular mass of approximately 25.2 kDa . It is ubiquitously expressed in various tissues, with the highest levels found in the heart, placenta, and skeletal muscle. Moderate levels are present in the lung and liver, while low levels are found in the brain, kidney, and pancreas .

RRAS2 cycles between an active GTP-bound state and an inactive GDP-bound state at the cytoplasmic face of the plasma membrane . It plays a crucial role in signal transduction pathways that control cell proliferation. RRAS2 is implicated in the pathogenesis of human cancers, as its overexpression can induce tumorigenicity through pathways such as phosphoinositide 3-kinase, p38 MAPK, and mTOR .

Mutations and overexpression of RRAS2 are associated with various cancers, including ovarian and breast cancer . The protein’s ability to transduce growth inhibitory signals across the cell membrane suggests it may have a role in counteracting the effects of other Ras proteins, potentially offering a target for therapeutic intervention .

The human recombinant RRAS2 is produced in Escherichia coli and is purified using proprietary chromatographic techniques . The recombinant protein is often used in research to study its role in cancer and other diseases.

The recombinant RRAS2 protein is typically stored at -20°C for long-term storage, with recommendations to add a carrier protein to prevent degradation. It is stable for short periods at 4°C if used within 2-4 weeks .