RRAGC Human

Ras-Related GTP Binding C Human Recombinant

Cat. No.

BT13176

Source

Escherichia Coli.

Synonyms

Ras-related GTP-binding protein C, Rag C, RagC, GTPase-interacting protein 2, TIB929, RRAGC, GTR2.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

RRAGC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 423 amino acids (1-399 a.a) and having a molecular mass of 46.7kDa.
RRAGC is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Ras-related GTP binding C (RRAGC) is a monomeric guanine nucleotide-binding protein, also known as a G protein. Small G proteins like RRAGC bind to GTP or GDP, acting as molecular regulators in various cellular processes and signaling pathways. Specifically, RRAGC regulates the organization of the actin cytoskeleton and possesses intrinsic GTPase activity. It is believed to be involved in the amino acid-induced relocalization of mTORC1 to lysosomes and its subsequent activation by the RHEB GTPase, a crucial step in activating the TOR signaling cascade by amino acids.

Description

Recombinant human RRAGC, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 423 amino acids (with amino acids 1-399 present) and possessing a molecular weight of 46.7 kDa. The RRAGC protein is fused to a 24 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

A clear solution that has been sterilized through filtration.

Formulation

The RRAGC protein solution is provided at a concentration of 0.5 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, and 0.1 M NaCl.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). It is important to avoid repeated freeze-thaw cycles to maintain product integrity.

Purity

The purity of the RRAGC protein is greater than 85.0% as determined by SDS-PAGE analysis.

Synonyms

Ras-related GTP-binding protein C, Rag C, RagC, GTPase-interacting protein 2, TIB929, RRAGC, GTR2.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSLQYG AEETPLAGSY GAADSFPKDF GYGVEEEEEE AAAAGGGVGA GAGGGCGPGG ADSSKPRILL MGLRRSGKSS IQKVVFHKMS PNETLFLEST NKIYKDDISN SSFVNFQIWD FPGQMDFFDP TFDYEMIFRG TGALIYVIDA QDDYMEALTR
LHITVSKAYK VNPDMNFEVF IHKVDGLSDD HKIETQRDIH QRANDDLADA GLEKLHLSFY LTSIYDHSIF EAFSKVVQKL IPQLPTLENL LNIFISNSGI EKAFLFDVVS KIYIATDSSP VDMQSYELCC DMIDVVIDVS CIYGLKEDGS GSAYDKESMA IIKLNNTTVL YLKEVTKFLA
LVCILREESF ERKGLIDYNF HCFRKAIHEV FEVGVTSHRS CGHQTSASSL KALTHNGTPR NAI.

Product Science Overview

Introduction

Ras-Related GTP Binding C (RRAGC) is a protein encoded by the RRAGC gene in humans. It belongs to the Ras superfamily of small GTPases, which are involved in various cellular processes, including cell growth, differentiation, and intracellular trafficking. RRAGC is particularly notable for its role in the regulation of the mechanistic target of rapamycin (mTOR) signaling pathway, which is crucial for cell growth and metabolism.

Structure and Function

RRAGC is a GTP-binding protein that cycles between an active GTP-bound state and an inactive GDP-bound state. This cycling is essential for its function as a molecular switch in signaling pathways. The protein is composed of several conserved domains that facilitate its interaction with other proteins and its ability to bind and hydrolyze GTP.

Role in mTOR Signaling

The mTOR signaling pathway is a central regulator of cell growth and metabolism, responding to various environmental cues such as nutrient availability, energy status, and growth factors. RRAGC, along with its binding partners RRAGA, RRAGB, and RRAGD, forms a heterodimeric complex that is crucial for the activation of mTORC1 (mechanistic target of rapamycin complex 1) in response to amino acids. This activation is essential for the proper regulation of protein synthesis, autophagy, and other metabolic processes.

Clinical Significance

Mutations or dysregulation of RRAGC have been associated with several diseases, including cancer and metabolic disorders. For instance, alterations in the mTOR signaling pathway, where RRAGC plays a pivotal role, have been implicated in the development and progression of various cancers. Additionally, RRAGC has been linked to rare genetic disorders such as Long-Olsen-Distelmaier Syndrome and Birt-Hogg-Dube Syndrome.

Therapeutic Potential

Given its central role in mTOR signaling, RRAGC is a potential target for therapeutic interventions. Inhibitors or modulators of RRAGC function could be developed to treat diseases associated with mTOR dysregulation, such as cancer and metabolic disorders. Research is ongoing to better understand the precise mechanisms by which RRAGC regulates mTOR signaling and to develop targeted therapies that can modulate its activity.

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RRAGC Human

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