RPS6KB1 Human
Sf9, Baculovirus cells.
Ribosomal protein S6 kinase beta-1 isoform a, p70 S6KA, p70(S6K)-alpha, p70-alpha, p70-S6K, PS6K, S6K, S6K-beta-1, S6K1, STK14A, 70 kDa ribosomal protein S6 kinase 1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA.
Greater than 85.0% as determined by SDS-PAGE.
Description
RPS6KB1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 533 amino acids (1-525a.a.) and having a molecular mass of 60.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
RPS6KB1 is expressed with a 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Ribosomal Protein S6 Kinase Beta-1 Isoform A, also known as RPS6KB1, is a serine/threonine kinase. This protein plays a role in the PI3 kinase pathway, specifically interacting with PIP3 and phosphoinositide-dependent kinase-1. RPS6KB1 is involved in protein synthesis by phosphorylating ribosomal protein S6. Historically, phosphorylation of p70S6K at threonine 389 has been linked to mTOR activation and autophagy inhibition. However, recent research suggests that p70S6K activity may actually promote autophagy.
Produced using Sf9 insect cells, our RPS6KB1 is a single, glycosylated polypeptide chain that contains 533 amino acids (specifically amino acids 1 through 525). This protein has a molecular weight of 60.2 kDa. Note: On SDS-PAGE, the molecular size will appear between 70-100 kDa. This RPS6KB1 protein features an 8 amino acid His tag located at the C-terminus. Purification is achieved through proprietary chromatographic techniques.
This 0.25mg/ml solution of RPS6KB1 protein is prepared with the following components: 50mM Tris-HCl (pH 7.5), 30% glycerol, 0.5M NaCl, 2mM DTT, and 0.1mM PMSF.
For long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised.
Repeated freezing and thawing should be avoided.
Analysis by SDS-PAGE confirms that the purity of this product exceeds 85%.
Ribosomal protein S6 kinase beta-1 isoform a, p70 S6KA, p70(S6K)-alpha, p70-alpha, p70-S6K, PS6K, S6K, S6K-beta-1, S6K1, STK14A, 70 kDa ribosomal protein S6 kinase 1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA.
Sf9, Baculovirus cells.
MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL
FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL
LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLLEHHH HHH.
Product Science Overview
Ribosomal Protein S6 Kinase B1 (RPS6KB1), also known as p70S6K, is a serine/threonine kinase that plays a crucial role in the regulation of cell growth, cell cycle progression, and protein synthesis. It is a member of the AGC (protein kinase A, G, and C) family of protein kinases and is regulated by the phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway .
RPS6KB1 was first identified in the late 1980s in the laboratory of George Thomas. It was detected in extracts of quiescent 3T3 cells that were stimulated to proliferate with epidermal growth factor or serum . The kinase is named for its ability to phosphorylate the ribosomal protein S6, which is a component of the 40S ribosomal subunit .
RPS6KB1 is activated by the PI3K/mTOR signaling pathway, which is a central regulator of cell survival, proliferation, and metabolism . Upon activation, RPS6KB1 phosphorylates the S6 protein of the ribosomal subunit 40S, leading to the selective translation of a unique family of mRNAs that contain an oligopyrimidine tract at the 5’ transcriptional site (5′TOP). These mRNAs encode components of the translational apparatus, including ribosomal proteins and elongation factors .
Due to its role in protein synthesis and cell growth, RPS6KB1 is involved in various human diseases, including cancer, diabetes, and obesity . It is considered a potential therapeutic target for drug development, particularly in the context of cancer treatment. Inhibitors of the mTOR pathway, which regulate RPS6KB1, are being explored for their anticancer and immunosuppressant effects .
Recent studies have suggested that plasma levels of RPS6KB1 can serve as a reliable biomarker for monitoring patient response to mTOR inhibitors . This has significant implications for the development of strategies to aid in the diagnosis, prognosis, and treatment of various pathological conditions.
