RPS3A Human

Ribosomal Protein S3A Human Recombinant
Cat. No.
BT17976
Source
E.coli.
Synonyms
Ribosomal protein S3A, v-fos transformation effector protein 1, 40S ribosomal protein S3a, MFTL, FTE1, S3A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPS3A Human Recombinant produced in E. coli is a single polypeptide chain containing 288 amino acids (1-264) and having a molecular mass of 32.5kDa.
RPS3A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RPS3A, belonging to the S3AE family of ribosomal proteins, is a crucial component of the smaller 40S subunit within ribosomes, the cellular machinery responsible for protein synthesis. Ribosomes themselves comprise a larger 60S and the smaller 40S subunit, with a collective composition of 4 RNA types and approximately 80 structurally diverse proteins. This protein is located in the cytoplasm. Studies have shown that disrupting the gene encoding rat ribosomal protein S3a in v-fos-transformed rat cells leads to a reversal of the transformed phenotype.
Description
Recombinantly produced in E. coli, the human RPS3A protein is a single polypeptide chain. It consists of 288 amino acids, with a sequence spanning from positions 1 to 264, and exhibits a molecular weight of 32.5 kDa. For purification purposes, a 24 amino acid His-tag is fused to the N-terminus of RPS3A. The protein is further purified using proprietary chromatographic methods.
Physical Appearance
The product is a clear, colorless solution that has been sterilized through filtration.
Formulation
The provided RPS3A solution has a concentration of 0.5 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 200mM NaCl, 2mM DTT, and 50% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided to maintain protein integrity.
Purity
SDS-PAGE analysis confirms a purity level exceeding 90%.
Synonyms
Ribosomal protein S3A, v-fos transformation effector protein 1, 40S ribosomal protein S3a, MFTL, FTE1, S3A.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAVGKN KRLTKGGKKG AKKKVVDPFS KKDWYDVKAP AMFNIRNIGK TLVTRTQGTK IASDGLKGRV FEVSLADLQN DEVAFRKFKL ITEDVQGKNC LTNFHGMDLT RDKMCSMVKK WQTMIEAHVD VKTTDGYLLR LFCVGFTKKR NNQIRKTSYA QHQQVRQIRK KMMEIMTREV QTNDLKEVVN KLIPDSIGKD IEKACQSIYP LHDVFVRKVK MLKKPKFELG KLMELHGEGS SSGKATGDET GAKVERADGY EPPVQESV.

Product Science Overview

Introduction

Ribosomal Protein S3A (RPS3A) is a crucial component of the ribosome, the cellular machinery responsible for protein synthesis. This protein belongs to the S3AE family of ribosomal proteins and is a part of the 40S subunit of the ribosome. The recombinant form of this protein, expressed in E. coli, is often used in research to study its structure and function.

Structure and Function

RPS3A is composed of 264 amino acids and has a predicted molecular mass of approximately 32.5 kDa . The protein is typically tagged with a His-tag at the N-terminus to facilitate purification. The amino acid sequence of RPS3A includes several regions that are crucial for its function in the ribosome.

The primary role of RPS3A is to contribute to the assembly and stability of the 40S ribosomal subunit. Ribosomes are composed of two subunits: the small 40S subunit and the large 60S subunit. Together, these subunits are made up of four RNA species and around 80 structurally distinct proteins .

Biological Significance

RPS3A is not only essential for protein synthesis but also plays a role in various cellular processes. Disruption of the gene encoding RPS3A in rat cells has been shown to revert the transformed phenotype of v-fos-transformed cells, indicating its role in cellular transformation and cancer .

Additionally, RPS3A has been implicated in several extraribosomal functions, including DNA repair, apoptosis, and the innate immune response to bacterial infection . These functions highlight the multifunctional nature of ribosomal proteins beyond their traditional role in protein synthesis.

Recombinant Expression

The recombinant form of RPS3A is produced by expressing the human RPS3A gene in E. coli. The protein is then purified using conventional chromatography techniques. The recombinant protein is often used in research to study its structure, function, and interactions with other cellular components .

Applications in Research

Recombinant RPS3A is widely used in various research applications, including:

  • Structural studies: Understanding the three-dimensional structure of RPS3A and its interactions with other ribosomal proteins and RNA.
  • Functional assays: Investigating the role of RPS3A in ribosome assembly, protein synthesis, and extraribosomal functions.
  • Disease research: Studying the involvement of RPS3A in cancer, DNA repair, and immune response to bacterial infections.

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