RPS3 Human

Ribosomal Protein S3 Human Recombinant
Cat. No.
BT17877
Source
E.coli.
Synonyms
S3, Ribosomal protein S3, 40S ribosomal protein S3, IMR-90 ribosomal protein S3, MGC87870, FLJ26283, FLJ27450.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPS3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (1-243) and having a molecular mass of 28.8 kDa.
RPS3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ribosomal protein S3, a member of the S3P family, plays a crucial role in protein synthesis and DNA repair. It facilitates mRNA binding during translation and participates in recognizing DNA damage, specifically abasic sites and oxidized guanine residues. RPS3 interacts with DNA repair proteins like OGG1 and Ref-1. Notably, RPS3 expression is elevated in colon cancer tissues, suggesting its potential involvement in tumor development.
Description
This product consists of a recombinant human RPS3 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 263 amino acids (residues 1-243) with a molecular weight of 28.8 kDa. The protein includes a 20 amino acid His-tag attached to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized through filtration.
Formulation
The RPS3 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of this product is greater than 90% as assessed by SDS-PAGE.
Synonyms
S3, Ribosomal protein S3, 40S ribosomal protein S3, IMR-90 ribosomal protein S3, MGC87870, FLJ26283, FLJ27450.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA.

Product Science Overview

Introduction

Ribosomal Protein S3 (RPS3) is a crucial component of the eukaryotic 40S ribosomal subunit. It plays a significant role in the process of protein synthesis by forming part of the domain where translation is initiated . Beyond its primary function in the ribosome, RPS3 has been found to have several extraribosomal roles, including involvement in DNA repair, apoptosis, and the innate immune response to bacterial infection .

Structure and Expression

RPS3 is a protein coding gene that belongs to the S3P family of ribosomal proteins. The human recombinant form of RPS3 is typically produced in Escherichia coli and consists of 263 amino acids, with a molecular mass of approximately 28.8 kDa . This recombinant protein is often fused to a His-tag at the N-terminus to facilitate purification .

Functions

  1. Ribosomal Function: As part of the 40S ribosomal subunit, RPS3 is essential for the initiation of translation. It binds to RNA and plays a role in the assembly of the ribosome, ensuring the accurate translation of mRNA into proteins .

  2. DNA Repair: RPS3 has been shown to function as an endonuclease involved in the repair of UV-induced DNA damage. This role is crucial for maintaining genomic stability and preventing mutations that could lead to diseases such as cancer .

  3. Apoptosis: RPS3 is involved in the regulation of apoptosis, the process of programmed cell death. This function is important for the removal of damaged or infected cells, thereby contributing to the overall health of the organism .

  4. Immune Response: RPS3 plays a role in the innate immune response to bacterial infection. It has been implicated in the regulation of pro-inflammatory signaling pathways, which are essential for the body’s defense against pathogens .

Clinical Relevance

Higher levels of RPS3 expression have been observed in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa . This suggests that RPS3 may be involved in the development or progression of certain types of cancer. Additionally, RPS3 has been implicated in various diseases, including febrile seizures and sex reversal conditions .

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