RPS2 Human

Ribosomal Protein S2 Human Recombinant
Cat. No.
BT17634
Source
Escherichia Coli.
Synonyms
Ribosomal Protein S2, Protein LLRep3, 40S Ribosomal Protein S4, 40S Ribosomal Protein S2, LLREP3, OK/KNS-cl.6, S2, RPS4.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPS2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (1-293aa) and having a molecular mass of 33.7kDa.
RPS2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ribosomal protein S2 (RPS2) belongs to the S5P family of ribosomal proteins. Ribosomes, responsible for protein synthesis, consist of a small 40S subunit and a large 60S subunit. These subunits comprise 4 RNA species and approximately 80 structurally distinct proteins. RPS2, a component of the 40S subunit, is located in the cytoplasm. Sharing sequence similarity with mouse LLRep3, RPS2 has been linked to diamond-blackfan anemia and herpes simplex.
Description
Recombinant human RPS2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 316 amino acids (1-293aa). It has a molecular mass of 33.7kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The RPS2 protein solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the RPS2 protein is determined to be greater than 85% using SDS-PAGE analysis.
Synonyms
Ribosomal Protein S2, Protein LLRep3, 40S Ribosomal Protein S4, 40S Ribosomal Protein S2, LLREP3, OK/KNS-cl.6, S2, RPS4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADDAGA AGGPGGPGGP GMGNRGGFRG GFGSGIRGRG RGRGRGRGRG RGARGGKAED KEWMPVTKLG RLVKDMKIKS LEEIYLFSLP IKESEIIDFF LGASLKDEVL KIMPVQKQTR AGQRTRFKAF VAIGDYNGHV GLGVKCSKEV ATAIRGAIIL AKLSIVPVRR GYWGNKIGKP HTVPCKVTGR CGSVLVRLIP APRGTGIVSA PVPKKLLMMA GIDDCYTSAR GCTATLGNFA KATFDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

Product Science Overview

Introduction

Ribosomal Protein S2 (RPS2) is a crucial component of the ribosome, the molecular machine responsible for protein synthesis in cells. In humans, ribosomes are composed of four ribosomal RNAs (rRNAs) and approximately 80 ribosomal proteins (RPs), including RPS2. The ribosome’s core composition is highly conserved across different species, reflecting its fundamental role in cellular life .

Structure and Function

RPS2 is a part of the small 40S subunit of the ribosome. It plays a vital role in the assembly and stability of the ribosome, as well as in the translation of messenger RNA (mRNA) into proteins. The ribosome’s function is essential for maintaining cellular homeostasis and supporting various physiological processes .

Variability and Expression

While the ribosome’s core structure is largely invariant, recent studies have revealed some degree of variability in ribosomal components, including RPS2. This variability can be observed in the expression levels of ribosomal proteins across different tissues and cell types. For instance, certain ribosomal proteins exhibit tissue-specific expression patterns, which can influence the translation of specific mRNAs .

Role in Disease

The expression of ribosomal proteins, including RPS2, can be dysregulated in various diseases, particularly cancer. Overexpression or mutations in ribosomal proteins have been linked to tumorigenesis and cancer progression. For example, RPS2 has been found to be overexpressed in malignant prostate cancer cell lines and tumor specimens, suggesting its potential role as a therapeutic target .

Recombinant Production

Human recombinant RPS2 is produced using recombinant DNA technology. This involves inserting the gene encoding RPS2 into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. The recombinant protein is then purified for use in research and therapeutic applications. Recombinant RPS2 is valuable for studying ribosome function, protein synthesis, and the role of ribosomal proteins in disease .

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