RPS24 Human

Ribosomal Protein S24 (RPS24) is a crucial component of the small 40S subunit of ribosomes, the organelles responsible for protein synthesis in cells . This protein belongs to the S24E family of ribosomal proteins and is located in the cytoplasm . The gene encoding RPS24 is known as RPS24 and is a protein-coding gene .

RPS24 plays a significant role in the assembly and function of the ribosome. It is required for the processing of pre-rRNA and the maturation of 40S ribosomal subunits . During the assembly of the small subunit (SSU) processome in the nucleolus, RPS24, along with other ribosomal proteins and biogenesis factors, associates with nascent pre-rRNA to facilitate RNA folding, modifications, rearrangements, and cleavage .

The RPS24 gene is located on chromosome 10 and has multiple transcript variants encoding different isoforms . As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS24 dispersed throughout the genome .

Mutations in the RPS24 gene are associated with Diamond-Blackfan Anemia (DBA), a rare congenital disorder characterized by failure of the bone marrow to produce red blood cells . Specifically, mutations in RPS24 can lead to Diamond-Blackfan Anemia 3 (DBA3) .

Recombinant Human RPS24 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the gene encoding RPS24 into a suitable expression system, such as E. coli, to produce the protein in large quantities . The recombinant protein often includes a His-tag at the N-terminus to facilitate purification .

Recombinant Human RPS24 is used in various research applications, including studies on ribosome function, protein synthesis, and genetic disorders like Diamond-Blackfan Anemia . It is also used in structural biology to understand the detailed architecture of ribosomal subunits .

Recombinant Human RPS24 is typically stored in a buffer solution containing Tris-HCl, NaCl, glycerol, and DTT to maintain its stability . It should be stored at 4°C for short-term use and at -20°C for long-term storage to avoid freeze-thaw cycles .