RPS20 Human

Ribosomal Protein S20 Human Recombinant

Cat. No.

BT17718

Source

Escherichia Coli.

Synonyms

S20, 40S ribosomal protein S20, RPS20.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

RPS20 Human Recombinant produced in E. coli is. a single polypeptide chain containing 165 amino acids (1-142) and having a molecular mass of 18.4kDa. RPS20 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Ribosomal Protein S20 (RPS20) is a protein that is found in ribosomes. Ribosomes are responsible for making proteins in the cell. RPS20 is found in the smaller of the two subunits of the ribosome, called the 40S subunit. This protein is part of a family of ribosomal proteins called the S10P family. RPS20 is located in the cytoplasm, which is the fluid inside of a cell. Interestingly, RPS20 is made at the same time as another type of RNA molecule called U54. U54 is a small nucleolar RNA, and its gene is located within the second intron of the RPS20 gene. Introns are parts of genes that are not directly involved in making proteins. There are multiple copies of the RPS20 gene that don't actually make functional proteins, these are called pseudogenes, and this is common for genes that encode ribosomal proteins.

Description

This product is a recombinant human RPS20 protein produced in E. coli bacteria. It is a single chain of 165 amino acids with a molecular weight of 18.4kDa. The protein sequence includes amino acids 1-142 of the natural human RPS20 protein. It also includes a 23 amino acid His-tag attached to the N-terminus to aid in purification. The protein is purified using proprietary chromatographic techniques.

Physical Appearance

A clear solution that has been sterilized by filtration.

Formulation

This solution contains RPS20 at a concentration of 0.25mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT, and 1mM EDTA.

Stability

For short-term storage (up to four weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product at -20°C. To further enhance stability during long-term storage, adding a carrier protein like HSA or BSA to a final concentration of 0.1% is recommended. It is important to avoid repeated freezing and thawing of the product.

Purity

The purity of this product is greater than 85% as determined by SDS-PAGE, a technique for separating proteins based on their size.

Synonyms

S20, 40S ribosomal protein S20, RPS20.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAFKDTG KTPVEPEVAI HRIRITLTSR NVKSLEKVCA DLIRGAKEKN LKVKGPVRMP TKTLRITTRK TPCGEGSKTW DRFQMRIHKR LIDLHSPSEI VKQITSISIE PGVELIESTD AEPMDTEGQQ YTLRSVFESP GTCPF.

Product Science Overview

Introduction

Ribosomal Protein S20 (RPS20) is a crucial component of the small 40S subunit of the ribosome, which plays a vital role in the synthesis of proteins within the cell. This protein is encoded by the RPS20 gene and is part of the S10P family of ribosomal proteins . The human recombinant form of RPS20 is produced through recombinant DNA technology, allowing for its use in various research and therapeutic applications.

Structure and Function

Ribosomes are complex molecular machines composed of ribosomal RNA (rRNA) and ribosomal proteins. They are responsible for translating messenger RNA (mRNA) into proteins. The small 40S subunit, which includes RPS20, binds to the mRNA and decodes its sequence, while the large 60S subunit catalyzes the formation of peptide bonds between amino acids.

RPS20 is one of the primary rRNA binding proteins. It binds directly to the 16S rRNA, nucleating the assembly of the bottom of the body of the 30S subunit by interacting with several RNA helices of the 16S rRNA. This interaction is essential for the proper assembly and function of the ribosome.

Gene and Protein Expression

The RPS20 gene is located on chromosome 8 in humans and is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS20 dispersed throughout the genome.

RPS20 is expressed in various tissues, including lymphoid tissue, bone marrow, testis, and skeletal muscle. Its expression is crucial for cell proliferation, protein ubiquitination, and the innate immune response.

Clinical Significance

Mutations in the RPS20 gene have been associated with several diseases, including Diamond-Blackfan Anemia and Familial Colorectal Cancer Type X. Diamond-Blackfan Anemia is a rare genetic disorder characterized by a failure to produce enough red blood cells, leading to anemia. Familial Colorectal Cancer Type X is a hereditary condition associated with an increased risk of developing colorectal cancer.

Applications of Human Recombinant RPS20

The human recombinant form of RPS20 is produced using recombinant DNA technology, which involves inserting the RPS20 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used in various research applications, including studies on ribosome assembly, protein synthesis, and the role of ribosomal proteins in disease.

Additionally, recombinant RPS20 can be used in therapeutic applications, such as the development of treatments for diseases associated with mutations in the RPS20 gene. By understanding the structure and function of RPS20, researchers can develop targeted therapies to correct or compensate for the effects of these mutations.

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RPS20 Human

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