RPS18 Human

Ribosomal Protein S18 Human Recombinant
Cat. No.
BT17487
Source
Escherichia Coli.
Synonyms
Ribosomal Protein S18, D6S218E, 40S Ribosomal Protein S18, HKE3, KE-3, KE3, S18, Rhabdomyosarcoma Antigen MU-RMS-40.21, Ke-3, Ke3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPS18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 175 amino acids (1-152 a.a) and having a molecular mass of 20.1kDa.
RPS18 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
40S ribosomal protein S18 (RPS18) is a member of the ribosomal protein S13P family. RPS18 is located in the cytoplasm. Ribosomes, the organelles which catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Jointly these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS18 encodes a ribosomal protein which is a component of the 40S subunit. RPS18 is a recognized binding partner of Cofilin and has been proposed to be a novel substrate for CaMKII. Among the diseases associated with RPS18 are Bowen-Conradi syndrome, and pasteurellosis.
Description
RPS18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 175 amino acids (1-152 a.a) and having a molecular mass of 20.1kDa. RPS18 is fused to a 23 amino acid His-tag at the N-terminus and purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered clear solution.
Formulation
RPS18 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea, and 10% glycerol.
Stability
Store at 4°C if the entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Ribosomal Protein S18, D6S218E, 40S Ribosomal Protein S18, HKE3, KE-3, KE3, S18, Rhabdomyosarcoma Antigen MU-RMS-40.21, Ke-3, Ke3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSLVIPE KFQHILRVLN TNIDGRRKIA FAITAIKGVG RRYAHVVLRK ADIDLTKRAG ELTEDEVERV ITIMQNPRQY KIPDWFLNRQ KDVKDGKYSQ VLANGLDNKL REDLERLKKI RAHRGLRHFW GLRVRGQHTK TTGRRGRTVG VSKKK

Product Science Overview

Introduction

Ribosomal Protein S18 (RPS18) is a crucial component of the ribosome, the cellular machinery responsible for protein synthesis. In humans, this protein is encoded by the RPS18 gene. The ribosome itself is composed of two subunits: the small 40S subunit and the large 60S subunit. RPS18 is a part of the 40S subunit and plays a significant role in the translation process.

Gene and Protein Structure

The RPS18 gene is located on chromosome 6 in humans . This gene belongs to the S13P family of ribosomal proteins and is highly conserved across different species, indicating its essential role in cellular function . The protein encoded by this gene is found in the cytoplasm and is involved in the binding of fMet-tRNA, which is crucial for the initiation of translation .

Function and Importance

Ribosomal proteins, including RPS18, are essential for the assembly and function of ribosomes. The 40S subunit, which includes RPS18, is responsible for decoding the mRNA and ensuring the correct alignment of tRNA and mRNA during protein synthesis . This process is vital for the accurate translation of genetic information into functional proteins.

Recombinant RPS18

Recombinant RPS18 refers to the protein that has been genetically engineered and produced in a laboratory setting. This is typically done by inserting the human RPS18 gene into a suitable expression system, such as bacteria or yeast, which then produces the protein. Recombinant proteins are invaluable in research and biotechnology, as they allow scientists to study the protein’s structure, function, and interactions in detail.

Applications in Research

Recombinant RPS18 is used in various research applications, including:

  • Structural studies: Understanding the three-dimensional structure of RPS18 helps in elucidating its role in the ribosome and its interactions with other ribosomal proteins and RNA.
  • Functional assays: Researchers use recombinant RPS18 to study its function in translation and its involvement in various cellular processes.
  • Drug development: By studying the interactions of RPS18 with other molecules, scientists can identify potential targets for therapeutic intervention in diseases related to protein synthesis.

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