RPS16 Human
Description
RPS16 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Ribosomal Protein S16 (RPS16) is a crucial component of the 40S subunit of the eukaryotic ribosome. It belongs to the S9P family of ribosomal proteins and is encoded by the RPS16 gene. This protein plays a vital role in the process of translation, where it contributes to the assembly and function of the ribosome, facilitating the synthesis of proteins from mRNA templates .
The RPS16 gene is located on chromosome 19 in humans and is characterized by multiple processed pseudogenes dispersed throughout the genome . The human recombinant RPS16 protein is typically produced in Escherichia coli (E. coli) and consists of a single, non-glycosylated polypeptide chain containing 169 amino acids, with a molecular mass of approximately 18.8 kDa . The recombinant protein often includes a His-tag at the N-terminus to facilitate purification .
The cDNA of human RPS16 is cloned into an expression vector, such as pET-15b, and expressed in E. coli cells . Large-scale production of the recombinant protein involves isolating highly purified protein from inclusion bodies. The refolding of the protein from these inclusion bodies is optimized to ensure proper structural stability . Techniques such as circular dichroism (CD) spectroscopy are used to analyze the secondary structure content of the refolded protein, revealing that approximately 21% of the amino acid sequence forms α-helices and 24% forms β-strands .
RPS16 is essential for the proper functioning of the ribosome. Its stability is influenced by various factors, including pH and urea concentration. The protein is quickly denatured at pH values above 8.0, while increasing urea concentration causes a slow unfolding of the protein . These properties are crucial for understanding the conditions under which RPS16 can maintain its functional integrity.
RPS16 is involved in several critical biological processes, including cell proliferation, immune response, and protein ubiquitination . It is expressed in various tissues, such as lymphoid tissue, bone marrow, testis, and skeletal muscle . The protein’s role in the ribosome makes it indispensable for the translation process, impacting overall cellular function and protein synthesis.
